Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish.
Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish.
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DOI：
10.1523/eneuro.0208-17.2017
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发表时间：
2018-01
期刊：
影响因子：
3.4
通讯作者：
Spence RD
中科院分区：
文献类型：
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作者：
Maheras AL;Dix B;Carmo OMS;Young AE;Gill VN;Sun JL;Booker AR;Thomason HA;Ibrahim AE;Stanislaw L;Dallego JC;Ngo CN;Chen A;Fortini BK;Spence RD
轻度创伤性脑损伤（mTBI）是最常见的神经系统疾病之一，人类在受伤后修复和再生中枢神经系统（CNS）组织的能力受到严重限制。然而，斑马鱼（Danio rerio）在成年后仍保持着进行完整和功能性神经再生的非凡能力。我们希望通过分析新型成年斑马鱼 mTBI 模型中的差异表达基因 (DEG) 来扩展对已知神经再生机制的了解。在这项研究中，将 mTBI 啮齿动物体重下降模型应用于成年斑马鱼。记忆测试显示 mTBI 组的空间记忆存在显着缺陷。我们通过 RNA 测序分析确定了损伤后 3 天和 21 天 (dpi) 的 DEG。所得 DEG 根据基因本体 (GO) 类别进行分类。在 3 dpi 时，GO 类别由峰值损伤反应途径组成。值得注意的是，在 21 dpi 时，GO 类别由神经再生途径组成。最终，这些结果验证了一种新型斑马鱼 mTBI 模型，并阐明了中枢神经系统损伤和神经再生中重要的 DEG。
Mild traumatic brain injuries (mTBIs) are one of the most prevalent neurological disorders, and humans are severely limited in their ability to repair and regenerate central nervous system (CNS) tissue postinjury. However, zebrafish (Danio rerio) maintain the remarkable ability to undergo complete and functional neuroregeneration as an adult. We wish to extend knowledge of the known mechanisms of neuroregeneration by analyzing the differentially expressed genes (DEGs) in a novel adult zebrafish model of mTBI. In this study, a rodent weight drop model of mTBI was adapted to the adult zebrafish. A memory test showed significant deficits in spatial memory in the mTBI group. We identified DEGs at 3 and 21 days postinjury (dpi) through RNA-sequencing analysis. The resulting DEGs were categorized according to gene ontology (GO) categories. At 3 dpi, GO categories consisted of peak injury response pathways. Significantly, at 21 dpi, GO categories consisted of neuroregeneration pathways. Ultimately, these results validate a novel zebrafish model of mTBI and elucidate significant DEGs of interest in CNS injury and neuroregeneration.