Mycoplasma hominis attaches to and locates intracellularly in human spermatozoa
Mycoplasma hominis attaches to and locates intracellularly in human spermatozoa
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DOI：
10.1093/humrep/del032
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发表时间：
2006-06-01
影响因子：
6.1
通讯作者：
Guerra-Infante, Fernando Martin
中科院分区：
文献类型：
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作者：
Diaz-Garcia, Francisco Javier;Herrera-Mendoza, Alma Patricia;Guerra-Infante, Fernando Martin
背景：精子 - 支原体相互作用的研究一直集中在感染对精子质量的影响上，但很少有研究报道这种细菌与精子的直接相互作用。
方法：从三名健康男性中选取有活力、可运动且无感染的精子群，与15 - 480感染复数（MOI）单位的DiIC(18)标记的人型支原体一起培养。在感染后10分钟到24小时之间，通过共聚焦显微镜以及伊红 - Y染料排斥试验对细胞进行分析。
结果：早在感染后10分钟，就可以看到人型支原体群附着在精子头部、中段或尾部。支原体与精子头部或尾部的相互作用比与中段的相互作用大约高2.5 - 4.5倍。对受感染精子的连续切片显示，人型支原体位于头部和中段区域的胞质空间内。一小部分受感染的精子显示出尾部弯曲或卷曲，和/或中段变粗。人型支原体感染未改变精子的活力。
结论：这些结果为人型支原体对人类精子细胞的附着和侵袭性提供了明确的证据，这似乎并不影响精子的活力，表明人型支原体与精子的短期相互作用导致不明显或细微的损伤，但可能对男性或夫妻的长期生育能力有影响。
BACKGROUND: The study of sperm-mycoplasma interaction has been focused on the effects of infection on sperm quality, but few studies have reported the direct interaction of this bacterium with spermatozoa. METHODS: Selected populations of viable, motile and infection-free human spermatozoa from three healthy men were incubated with 15-480 multiplicity of infection (MOI) units of DiIC(18)-labelled Mycoplasma hominis. Cells were analyzed by means of confocal microscopy and by the eosin-Y dye exclusion test between 10 min and 24 h post-infection. RESULTS: As early as 10 min post-infection, clusters of M. hominis were seen attached to the sperm head, midpiece or tail. Mycoplasma showed an approximately 2.5-4.5-fold higher interaction with sperm head or tail than with midpiece. Sequential sectioning of infected spermatozoa revealed the intracellular location of M. hominis within cytosolic spaces of head and midpiece regions. A minor proportion of infected spermatozoa showed bent or coiled tails, and/or midpiece thickening. Sperm viability was not altered by M. hominis infection. CONCLUSIONS: These results provide specific and conclusive evidence of M. hominis attachment and invasiveness towards human sperm cells, which seems not to affect their viability, suggesting that a short-term M. hominis interaction with spermatozoa results in non-apparent or subtle damage, but might have implications for long-term male or couple's fertility.