A novel effector caspase (Caspase-3/7-1) involved in the regulation of immune homeostasis in Chinese mitten crab Eriocheir sinensis
A novel effector caspase (Caspase-3/7-1) involved in the regulation of immune homeostasis in Chinese mitten crab Eriocheir sinensis
复制标题
一种参与中华绒螯蟹免疫稳态调节的新型效应半胱天冬酶（Caspase-3/7-1）
DOI：
10.1016/j.fsi.2018.09.013
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发表时间：
2018
影响因子：
4.7
通讯作者：
Song Linsheng
中科院分区：
文献类型：
--
作者：
Qu Chen;Yang Wen;Xu Qingsong;Sun Jiejie;Lu Mengmeng;Wang Ying;Liu Chao;Wang Weilin;Wang Lingling;Song Linsheng
Caspases are a conserved family of cysteine proteases characterized by specificity for aspartic acid and play an essential role in cell apoptosis. In the present study, a novel effector caspase (designated asEsCaspase-3/7-1) was identified from Chinese mitten crabEriocheir sinensis. The open reading frame ofEsCaspase-3/7-1 cDNA was of 972 bp, encoding a polypeptide of 323 amino acids.EsCaspase-3/7-1 contained an N-terminal prodomain and a conservative C-terminal CASc domain, with the conserved active site “QACRG”. The mRNA transcripts ofEsCaspase-3/7-1 were constitutively expressed in all the examined tissues with high expression level in hemocytes, hepatopancreas and gill. TheEsCaspase-3/7-1 protein was mainly distributed in the cytoplasm of hemocytes. After Aeromonas hydrophila and lipopolysaccharide (LPS) stimulations, the mRNA expression level ofEsCaspase-3/7-1 in hemocytes increased significantly. The mRNA expression level ofEsCaspase-3/7-1 in hemocytes was significantly up-regulated after H2O2treatmentin vitro. The recombinantEsCaspase-3/7-1 protein (rEsCaspase-3/7-1) was capable of hydrolyzing the substrate Ac-DEVD-pNA rather than Ac-YVAD-pNA and Ac-VEID-pNAin vitro, and exhibited binding activity to LPS. These results demonstrated thatEsCaspase-3/7-1 might act as an LPS receptor, and play an important role in the regulation of immune homeostasis ofE. sinensis.
