Heterologous expression of a Tpo1 homolog from Arabidopsis thaliana confers resistance to the herbicide 2,4-D and other chemical stresses in yeast
Heterologous expression of a Tpo1 homolog from Arabidopsis thaliana confers resistance to the herbicide 2,4-D and other chemical stresses in yeast
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DOI：
10.1007/s00253-009-2025-5
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发表时间：
2009-10-01
影响因子：
5
通讯作者：
Sa-Correia, Isabel
中科院分区：
文献类型：
--
作者：
Cabrito, Tania R.;Teixeira, Miguel C.;Sa-Correia, Isabel
了解获得性除草剂抗性的分子机制对于处理抗性杂草的出现至关重要。酿酒酵母已被用作一个模型系统，以深入了解潜在的抗除草剂2，4-二氯苯氧乙酸（2，4-D）的机制。TPO 1基因编码主要易化超家族（MFS）的多药耐药（MDR）质膜转运蛋白，先前发现该基因在酵母中赋予对2，4-D的抗性，并在响应除草剂时被转录激活。在这项工作中，我们证明，Tpo 1 p是需要降低细胞内浓度的2，4-D。ScTpo 1 p同源物编码假定的质膜MFS转运蛋白从植物模型拟南芥进行了分析，在2，4-D抗性的可能作用。选择At 5g 13750进行进一步分析，因为发现其转录水平在2，4-D胁迫的植物中增加。发现该植物开放阅读框在酵母中的功能性异源表达通过降低细胞内2，4-D浓度而赋予Delta tpo 1和野生型细胞对除草剂的抗性增加。At 5g 13750在酵母中的异源表达还导致对吲哚-3-乙酸（IAA）、Al 3+和Tl 3+的抗性增加。At 5g 13750是第一个被认为可能参与MDR的植物MFS转运蛋白。
The understanding of the molecular mechanisms underlying acquired herbicide resistance is crucial in dealing with the emergence of resistant weeds. Saccharomyces cerevisiae has been used as a model system to gain insights into the mechanisms underlying resistance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The TPO1 gene, encoding a multidrug resistance (MDR) plasma membrane transporter of the major facilitator superfamily (MFS), was previously found to confer resistance to 2,4-D in yeast and to be transcriptionally activated in response to the herbicide. In this work, we demonstrate that Tpo1p is required to reduce the intracellular concentration of 2,4-D. ScTpo1p homologs encoding putative plasma membrane MFS transporters from the plant model Arabidopsis thaliana were analyzed for a possible role in 2,4-D resistance. At5g13750 was chosen for further analysis, as its transcript levels were found to increase in 2,4-D stressed plants. The functional heterologous expression of this plant open reading frame in yeast was found to confer increased resistance to the herbicide in Delta tpo1 and wild-type cells, through the reduction of the intracellular concentration of 2,4-D. Heterologous expression of At5g13750 in yeast also leads to increased resistance to indole-3-acetic acid (IAA), Al3+ and Tl3+. At5g13750 is the first plant putative MFS transporter to be suggested as possibly involved in MDR.