Excretion of metabolites by hydrogen bacteria
Excretion of metabolites by hydrogen bacteria
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氢细菌代谢产物的排泄
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
A. Janczikowski
中科院分区：
文献类型：
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作者：
D. Vollbrecht;H. Schlegel;G. Stoschek;A. Janczikowski
研究了真养产碱杆菌野生型和突变株的代谢产物和聚-3-羟基丁酸的呼吸速率依赖性形成。在限制呼吸期间排泄几种代谢物，每种代谢物以不同的相对呼吸速率排泄。相对呼吸速率定义为生产阶段期间的呼吸速率与指数生长阶段结束时的呼吸速率之比（百分比）。排泄了以下代谢产物：乙醇、1-丙醇和2-甲基-1-丙醇为2.4，D，L-2，3-丁二醇为4.4，内消旋-2，3-丁二醇为5.1，乙偶姻为4.4至5.5，2-甲基丙酸酯、2-甲基丁酸酯和3-甲基丁酸酯为5至6，琥珀酸酯为5.5，甲酸酯为6.7，分子氢为5至7，乙酸酯为7.2，丁酸酯7.4，3-羟基丁酸酯10 - 11，顺乌头酸酯20.6，2-氧代-3-甲基丁酸酯26.5，2-氧代戊二酸酯约50%。聚-3-羟基丁酸酯的最大形成发生在40至50%。当呼吸速率不受限制时，底物被完全氧化。代谢产物的排泄是反映细胞氧营养状况的重要指标，呼吸速率与氧的利用率呈正相关，与葡萄糖酸盐的利用率呈负相关。加入葡萄糖酸盐（1.5%）后，呼吸速率立即降低。
SummaryThe respiration rate-dependent formation of metabolites and of poly-3-hydroxybutanoate was studied with wild type and mutant strains of Alcaligenes eutrophus. Several metabolites were excreted during restricted respiration, each metabolite at a distinct relative respiration rate. The relative respiration rate was defined as the ratio (in percent) of the respiration rate during the production phase to that at the end of the exponential growth phase. The following metabolites were excreted: Ethanol, 1-propanol and 2-methyl-1-propanol at 2.4, D,L-2,3-butanediol at 4.4, meso-2,3-butanediol at 5.1, acetoin at 4.4 to 5.5, 2-methylpropanoate, 2-methylbutanoate and 3-methylbutanoate at 5 to 6, succinate at 5.5, formate at 6.7, molecular hydrogen at 5 to 7, acetate at 7.2, butanoate at 7.4, 3-hydroxybutanoate at 10 to 11, cis-aconitate at 20.6, 2-oxo-3-methylbutanoate at 26.5, and 2-oxoglutarate at about 50%. The maximum formation of poly-3-hydroxybutanoate occurred at 40 to 50%. When the respiration rate was not restricted, the substrate was completely oxidized. The excretion of metabolites is a very useful indicator for the oxygen nutrition of the cells.The respiration rate correlated positively with the availability of oxygen and negatively with that of gluconate. Upon addition of gluconate (1.5%), the respiration rate immediately decreased.