THE RAM - A NOVEL LOW-MOLECULAR-WEIGHT GTP-BINDING PROTEIN CDNA FROM A RAT MEGAKARYOCYTE LIBRARY
THE RAM - A NOVEL LOW-MOLECULAR-WEIGHT GTP-BINDING PROTEIN CDNA FROM A RAT MEGAKARYOCYTE LIBRARY
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DOI：
10.1016/0014-5793(90)81431-m
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发表时间：
1990-11-26
期刊：
影响因子：
3.5
通讯作者：
NOZAWA, Y
中科院分区：
文献类型：
--
作者：
NAGATA, K;SATOH, T;NOZAWA, Y
用合成的寡核苷酸探针从大鼠巨核细胞cDNA文库中分离了一种新的低MrGTP结合蛋白cDNA，该探针对应于对c25 KG特异的8个氨基酸序列，c25 KG是一种先前从人血小板胞质溶胶组分中分离的GTP结合蛋白[（1989）J. Biol. Chem. 264，17000-17005]。该cDNA具有一个开放阅读框架，编码221个氨基酸的蛋白质，计算的Mr为25068。该蛋白被命名为asram（ras相关基因来自巨核细胞）蛋白（ramp 25）。从theramcDNA推导的氨基酸序列包含GTP结合和GTP酶域的共有序列。ramp 25与H‐ras、smg 25 A和c25 KG蛋白分别具有约23%、39%和80%的氨基酸同源性。在脾细胞中大量检测到3.5-kbrammRNA。
A novel lowMrGTP‐binding protein cDNA was isolated from a rat megakaryocyte cDNA library with a synthetic oligonucleotide probe corresponding to an 8‐amino acid sequence specific for c25KG, a GTP‐binding protein previously isolated from human platelet cytosol fraction [(1989) J. Biol. Chem. 264, 17000–17005]. The cDNA has an open reading frame encoding a protein of 221 amino acids with a calculatedMrof 25068. The protein is designated asram(ras‐related gene from megakaryocyte) protein (ramp25). The amino acid sequence deduced from theramcDNA contains the consensus sequences for GTP‐binding and GTPase domains.ramp25 shares about 23%, 39% and 80% amino acid homology with the H‐ras,smg25A and c25KG proteins, respectively. The 3.5‐kbrammRNA was detected abundantly in spleen cells.