In vitro ultraviolet A irradiation decreases both release ability and gene‐expression of vascular endothelial growth factor‐A from mast cells
In vitro ultraviolet A irradiation decreases both release ability and gene‐expression of vascular endothelial growth factor‐A from mast cells
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体外紫外线 A 照射降低肥大细胞血管内皮生长因子-A 的释放能力和基因表达
DOI：
10.1111/j.1600-0781.2012.00652.x
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
G. Monfrecola
中科院分区：
文献类型：
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作者：
G. Delfino;L. Di Costanzo;A. Paulis;G. Fabbrocini;G. Monfrecola
紫外线A(UVA)穿透真皮，影响包括肥大细胞在内的不同细胞的功能，能够产生血管生成因子。我们研究了体外培养的肥大细胞经长波紫外线照射后，血管内皮生长因子(VEGF)的释放和基因表达。用钙离子载体A23187和佛波酯(PMA)诱导人MCS-1(HMC-1)释放血管内皮生长因子-A(VEGF-A)。半数细胞接受剂量递增的长波紫外线(5、25和50 J/cm~2)，未照射的HMC-1细胞作为对照。采用酶联免疫吸附试验(ELISA法)和逆转录聚合酶链式反应(RT-PCR)法分别检测血管内皮生长因子释放和信使核糖核酸(MRNA)的表达。结果表明，长波紫外线对HMC-1释放血管内皮生长因子-A的抑制作用呈剂量依赖性，其中5 J/cm~2组、25 J/cm~2组和50 J/cm~2组的释放能力分别下降71.2%、85%和86.3%。
Ultraviolet A (UVA) rays penetrate the dermis, influencing the function of different cells, including mast cells, able to produce angiogenic factors. We investigated vascular endothelial growth factor (VEGF) release and gene‐expression from mast cells (MCs), after UVA irradiation in vitro. The release of VEGF‐A by Human MCs‐1 (HMC‐1) was induced by calcium ionophore A23187 and phorbol 12 myristate 13 acetate (PMA). Half of the cells received increasing doses of UVA (5, 25 and 50 J/cm2), the unirradiated HMC‐1 served as controls. VEGF release and VEGF‘s messenger ribonucleic acid (mRNA) were detected respectively by enzyme‐linked immunoabsorbent assay (ELISA) and reverse transcription‐polymerase chain reaction (RT‐PCR) analysis. Results showed a UVA dose‐dependent inhibitory effect on VEGF‐A release from HMC‐1. In particular, the release ability was reduced by 71.2% with 5 J/cm2; 85% with 25 J/cm2 and 86.3% with 50 J/cm2.