A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and Polydopamine
A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and Polydopamine
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DOI：
10.1007/s42235-022-00191-3
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发表时间：
2022-06
影响因子：
4
通讯作者：
Peng Bai;Gangcheng Kong;Weihua Qiao;Yu Song;Yixuan Wang;Jiawei Shi;N. Zhang;Chungeng Liu;Chong Chu;Tixiusi Xiong;Ying Zhou;Cuifen Lu;Lin Wang;N. Dong
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文献类型：
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作者：
Peng Bai;Gangcheng Kong;Weihua Qiao;Yu Song;Yixuan Wang;Jiawei Shi;N. Zhang;Chungeng Liu;Chong Chu;Tixiusi Xiong;Ying Zhou;Cuifen Lu;Lin Wang;N. Dong
心脏瓣膜置换术是治疗心脏瓣膜病的有效方法。临床常规使用的人工瓣膜仍存在缺陷。在我们的研究中，我们探索了一种新的方法来改善脱细胞心脏瓣膜（DHV）支架的性能。采用连续增溶法和亲脂增溶法制备脱细胞猪主动脉瓣。采用溴化锂法从缺乏丝素蛋白的蚕茧中提取丝胶蛋白。首先，将DHV浸泡在丝胶溶液中，制备出丝胶-DHV复合支架。然后，我们通过首先在DHV上制备聚多巴胺（PDA）涂层，然后结合丝胶蛋白来修饰DHV。通过体外和体内实验对复合支架的物理性能和生物相容性进行了评价。成功制备了丝胶蛋白，并将其与DHV复合，提高了DHV的生物相容性。PDA涂层进一步促进了DHV与丝胶的结合，改善了支架的物理性能。改良后的瓣膜支架在体内的降解率降低，与血液相容性良好。总之，我们的改性改善了瓣膜支架的物理性能和生物相容性。PDA和丝胶蛋白的结合促进了脱细胞瓣膜的再细胞化，显示出作为新型人工瓣膜的巨大潜力。
Cardiac valve replacement is an effective method to treat valvular heart disease. Artificial valves used routinely in clinic still have defects. In our study, we explored a novel method to modify the performance of Decellularized Heart Valve (DHV) scaffold. The decellularized porcine aortic valve was prepared using sequential hydrophile and lipophile solubilization method. The sericin was extracted from silk fibroin-deficient silkworm cocoon by lithium bromide method. First, DHV was immersed in sericin solution to produce the sericin–DHV composite scaffold. Then, we modified the DHV by making a Polydopamine (PDA) coating on the DHV first and then binding the sericin. The physical properties and biological compatibility of our composite scaffold were assessed in vitro and in vivo. Sericin were successfully prepared, combined to DHV and improved its biocompatibility. PDA coating further promoted the combination of sericin on DHV and improved the physical properties of scaffolds. The decay rate of our modified valve scaffold was decreased in vivo and it showed good compatibility with blood. In conclusion, our modification improved the physical properties and biocompatibility of the valve scaffold. The combination of PDA and sericin promoted the recellularization of decellularized valves, showing great potential to be a novel artificial valve.