The virion host shutoff protein of herpes simplex virus inhibits reporter gene expression in the absence of other viral gene products.
The virion host shutoff protein of herpes simplex virus inhibits reporter gene expression in the absence of other viral gene products.
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单纯疱疹病毒的病毒体宿主关闭蛋白在没有其他病毒基因产物的情况下抑制报告基因表达。
DOI：
10.1006/viro.1995.1431
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Read,GS
中科院分区：
文献类型：
--
作者：
Pak,AS;Everly,DN;Knight,K;Read,GS
单纯疱疹病毒的病毒体宿主关闭（vhs）功能诱导宿主mRNA在早期降解和整个感染过程中重要mRNA的快速周转。以前的研究表明，破坏UL41基因消除vhs活性，但没有确定UL41多肽是否是mRNA降解的直接诱导剂，或者它是否是该活性所需的唯一病毒体组分。在本文中，我们报告说，转染细胞与UL 41抑制共转染的CAT报告基因的表达，并且这种抑制不依赖于其他病毒基因。CAT表达的抑制是由于CAT mRNA水平的UL41依赖性降低。编码在病毒感染期间缺乏vhs活性的多肽的UL41等位基因在转染的细胞中表现出类似的活性缺乏。结果表明，UL41多肽是病毒感染后宿主mRNA降解的直接诱导物，并且它是该活性直接需要的唯一病毒体组分。缺失UL41的最后107个残基的382个氨基酸的无义多肽缺乏抑制活性，但被包装到病毒体中，而343个氨基酸的无义多肽既缺乏抑制活性又缺乏被包装的能力。
The virion host shutoff (vhs) function of herpes simplex virus induces degradation of host mRNAs at early times and rapid turnover of vital mRNAs throughout infection. Previous studies have shown that disruption of the UL41 gene abrogates vhs activity, but have not determined whether the UL41 polypeptide is the direct inducer of mRNA degradation or whether it is the only virion component required for this activity. In this paper we report that transfaction of cells with UL41 inhibits expression of a cotransfected CAT reporter gene and that the inhibition is not dependent upon other viral genes. Inhibition of CAT expression was due to UL41-dependent reduction of CAT mRNA levels. UL41 alleles encoding polypeptides that lacked vhs activity during virus infections exhibited a similar lack of activity in transfected cells. The results indicate that the UL41 polypeptide is the direct inducer of host mRNA degradation following virus infection and that it is the only virion component directly required for this activity. A 382-amino-acid nonsense polypeptide missing the last 107 residues of UL41 lacked inhibitory activity, but was packaged into virions, while a 343-amino-acid nonsense polypeptide lacked both inhibitory activity and the ability to be packaged.