DNA characterization of simian Entamoeba histolytica-like strains to differentiate them from Entamoeba histolytica
DNA characterization of simian Entamoeba histolytica-like strains to differentiate them from Entamoeba histolytica
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DOI：
10.1007/s00436-009-1480-3
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发表时间：
2009-05
影响因子：
2
通讯作者：
Jun-ichiro Takano;H. Tachibana;Miyoko Kato;Toyoko Narita;T. Yanagi;Y. Yasutomi;K. Fujimoto
中科院分区：
文献类型：
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作者：
Jun-ichiro Takano;H. Tachibana;Miyoko Kato;Toyoko Narita;T. Yanagi;Y. Yasutomi;K. Fujimoto
两个类溶组织内阿米巴（Entamoeba histolytica like）菌株EHMfas 1和P19-061405已被认为是基于遗传特征的一个新种。己糖激酶，磷酸葡萄糖异构酶，磷酸葡萄糖变位酶基因的序列分析支持了先前的同工酶分析结果，证明了一种新的酶模式。18 S rDNA，5.8S rDNA，伴侣蛋白60基因，和吡啶核苷酸转氢酶基因的系统发育研究表明，原始的simianE集群。溶组织样菌株低于或接近E. histolytica，分别几丁质酶和富含丝氨酸的大肠杆菌的比较研究。溶组织蛋白基因和基因座1-2区域揭示了大多数突变单元在猿类中是共享的。溶组织样菌株。猿猴体内每个重复单元的相似性。溶组织样菌株或E.两者之间的差异可能是协同进化的结果。我们的研究结果表明，EHMfas 1和P19-061405应该被认为是同一物种，尽管它们是从不同的猴子物种和不同的栖息地分离的。SimianE.溶组织样阿米巴可能是猕猴特有，与E相似。histolyticainhumans，应弗罗姆.如P19-061405所述，可溶解组织，复苏名称为纳氏内阿米巴。
Two simianEntamoeba histolytica-like strains, EHMfas1 and P19-061405, have been suggested to represent a new species based on genetic characterization. Sequence analyses of the hexokinase, glucose phosphate isomerase, and phosphoglucomutase genes supported the previous findings of isoenzyme analyses demonstrating a new zymodeme pattern. Phylogenetic studies of 18S rDNA, 5.8S rDNA, the chaperonin 60 gene, and the pyridine nucleotide transhydrogenase gene showed original clusters of simianE. histolytica-like strains below or nearE. histolytica, respectively. Comparative studies of the chitinase and the serine-richE. histolyticaprotein genes and locus 1–2 region revealed that most mutated units were shared among the simianE. histolytica-like strains. The similarities of each of the repeating units within the simianE. histolytica-like strains orE. histolyticaand the differences of those between the both might be generated by concerted evolution. Our results indicate that EHMfas1 and P19-061405 should be considered to be the same species, despite that they were isolated from different monkey species and different habitats. SimianE. histolytica-like amebas may be endemic to macaque monkeys, as a counterpart toE. histolyticain humans, and should be differentiated fromE. histolyticaby the revival nameEntamoeba nuttalli, as proposed for P19-061405.