A Pseudomonas aeruginosa quorum-sensing molecule influences Candida albicans morphology
A Pseudomonas aeruginosa quorum-sensing molecule influences Candida albicans morphology
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DOI：
10.1111/j.1365-2958.2004.04349.x
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发表时间：
2004-12-01
影响因子：
3.6
通讯作者：
Kolter, R
中科院分区：
文献类型：
--
作者：
Hogan, DA;Vik, Å;Kolter, R
白色念珠菌是一种机会性病原体，通常作为人类微生物群的成员被发现。白色念珠菌改变其细胞形态的能力与其毒力有关。酵母细胞在共生相互作用中更为普遍，而丝状细胞在机会性感染中似乎很重要。白色念珠菌在宿主环境中遇到多种其他微生物物种，它们很可能影响白色念珠菌在有毒和无毒状态之间的转变。在这里，我们报告白色念珠菌的形态受到另一种机会性病原体铜绿假单胞菌的存在的显着影响。在使用白色念珠菌 HWP1-lacZ 菌株来指示丝状生长区域的筛选中，我们鉴定了无法抑制白色念珠菌丝状形成的铜绿假单胞菌突变体。通过这些研究，我们发现 3-oxo-C12 高丝氨酸内酯（铜绿假单胞菌产生的一种细胞间信号分子）足以抑制白色念珠菌丝状化，而不影响真菌生长速率。形态特异性标记的显微镜分析和实时逆转录聚合酶链反应分析均证实，200 μM 3-oxo-C12 高丝氨酸内酯抑制丝化。具有 12 个碳链长度的结构相关化合物，例如C12-酰基高丝氨酸内酯和十二烷醇在相似浓度下也影响白色念珠菌丝状化。相比之下，其他不同链长的酰化高丝氨酸内酯不影响真菌形态。将 3OC12HSL 的活性与法尼醇（一种白色念珠菌产生的也具有 C12 主链的分子）的活性进行了比较。细菌对白色念珠菌形态的影响是细菌影响真菌细胞行为的方式之一。
Candida albicans is an opportunistic pathogen that is commonly found as a member of the human microflora. The ability of C. albicans to alter its cellular morphology has been associated with its virulence; yeast cells are more prevalent in commensal interactions whereas filamentous cells appear important in opportunistic infections. C. albicans encounters a multitude of other microbial species in the host environment and it is likely that they impact the C. albicans transition between virulent and non-virulent states. Here, we report that C. albicans morphology is significantly affected by the presence of Pseudomonas aeruginosa, another opportunistic pathogen. In a screen using a C. albicans HWP1-lacZ strain to indicate regions of filamentous growth, we identified P. aeruginosa mutants incapable of inhibiting C. albicans filamentation. Through these studies, we found that 3-oxo-C12 homoserine lactone, a cell-cell signalling molecule produced by P. aeruginosa, was sufficient to inhibit C. albicans filamentation without affecting fungal growth rates. Both microscopic analysis and real-time reverse transcription polymerase chain reaction analysis of morphology-specific markers confirmed that filamentation was suppressed by 200 muM 3-oxo-C12 homoserine lactone. Structurally related compounds with a 12-carbon chain length, e.g. C12-acyl homoserine lactone and dodecanol also affected C. albicans filamentation at similar concentrations. In contrast, other acylated homoserine lactones of different chain lengths did not affect fungal morphology. The activity of 3OC12HSL is compared with that of farnesol, a C. albicans-produced molecule also with a C12-backbone. The effects that bacteria have on the morphology of C. albicans represents one of the ways by which bacteria can influence the behaviour of fungal cells.