The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP.
The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP.
复制标题
c-fos 血清反应元件对蛋白激酶 C 依赖性和非依赖性信号产生反应，但对环 AMP 不产生反应。
DOI：
10.1101/gad.2.4.394
复制
发表时间：
1988
影响因子：
10.5
通讯作者：
Gilman,MZ
中科院分区：
文献类型：
--
作者：
Gilman,MZ
c-fos原癌基因的转录被血清生长因子快速诱导。一个短的c-fos DNA元件，血清反应元件（SRE），需要这种对血清的反应。然而，血清激活一系列不同的细胞内信号传导途径，并且不知道SRE响应于这些途径中的哪一个。为了解决这个问题，突变已被引入到一个否则完整的c-fos启动子/增强子的SRE。这些突变强烈地减少了核因子与该位点的结合。将携带野生型或突变型c-fos SRE的质粒转染到成纤维细胞中，并测试它们对全血清、纯化的重组c-sis蛋白、蛋白激酶C激活剂佛波醇肉豆蔻酸酯乙酸酯和环AMP（cAMP）第二信使系统激活剂的反应。在正常条件下和长期佛波酯处理后进行测定，以耗尽转染细胞中佛波酯可激活的蛋白激酶C活性。结果表明，SRE是必要的和足够的蛋白激酶C依赖和非依赖的细胞内信号通路的反应，但不是响应cAMP途径。
Transcription of the c-fos proto-oncogene is rapidly induced by serum growth factors. A short c-fos DNA element, the serum response element (SRE), is required for this response to serum. However, serum activates a series of distinct intracellular signaling pathways, and it is not known to which of these pathways the SRE responds. To address this question, mutations have been introduced into the SRE of an otherwise intact c-fos promoter/enhancer. These mutations strongly reduce the binding of a nuclear factor to this site. Plasmids carrying either a wild-type or mutant c-fos SRE were transfected into fibroblasts and tested for their response to whole serum, purified recombinant c-sis protein, the protein kinase C activator phorbol myristate acetate, and activators of the cyclic AMP (cAMP) second messenger system. Assays were carried out under normal conditions and after chronic phorbol ester-treatment to deplete phorbol ester activatable protein kinase C activity from transfected cells. The results show that the SRE is necessary and sufficient for response to both protein kinase C-dependent and -independent intracellular signaling pathways but not for response to the cAMP pathway.