ELECTRON CRYOMICROSCOPY AND ANGULAR RECONSTITUTION USED TO VISUALIZE THE SKELETAL-MUSCLE CALCIUM-RELEASE CHANNEL
ELECTRON CRYOMICROSCOPY AND ANGULAR RECONSTITUTION USED TO VISUALIZE THE SKELETAL-MUSCLE CALCIUM-RELEASE CHANNEL
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DOI：
10.1038/nsb0195-18
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
VANHEEL, M
中科院分区：
文献类型：
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作者：
SERYSHEVA, II;ORLOVA, EV;VANHEEL, M
我们利用的随机取向的冰嵌入式分子成像的电子冷冻显微镜，以确定在其封闭状态下的肌浆网（SR）的钙释放通道的三维结构，而不倾斜的标本保持器。我们的新的重建方法包括一个详尽的搜索所有不同的特征投影图像的显微照片和分配的欧拉角方向，这些意见。所暗示的30埃图揭示了其中跨膜区在SR腔侧上没有表现出明显开口的结构。延伸的胞质区域具有中空的外观，并且在每个单体中由钳形结构域和钳形结构域组成。
We exploit the random orientations of ice-embedded molecules imaged in an electron cryomicroscope to determine the three-dimensional structure of the Ca2+-release channel from the sarcoplasmic reticulum (SR) in its closed state, without tilting the specimen holder. Our new reconstruction approach includes an exhaustive search of all different characteristic projection Images in the micrographs anti the assignment of Euler angle orientations to these views. The 30 Angstrom map implied reveals a structure in which the transmembrane region exhibits no apparent opening on the SR lumen side. The extended cytoplasmic region has a hollow appearance and consists, in each monomer, of a clamp-shapen and a handle-shaped domain.