Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate
复制标题
用硫酸角质素单克隆抗体鉴定出两个分化软骨细胞亚群
作者：
M. Zanetti;A. Ratcliffe;F. Watt
我们制备了一种能与硫酸角蛋白特异结合的单抗，命名为MZ15。免疫荧光研究表明，硫酸角蛋白在关节软骨中的分布并不均匀：随着距离关节表面的距离增加，硫酸角蛋白的含量增加。从软骨分离后，通过细胞表面角蛋白硫酸盐的存在或不存在可以区分两种软骨细胞亚群。角质形成蛋白阴性的软骨细胞来自上软骨层。因此，软骨基质的生化异质性与软骨细胞群体中的异质性直接相关。在单层培养的过程中，浅层软骨细胞开始合成硫酸角蛋白，但仍可与深层或未分离软骨细胞的培养区分开来，因为它们的基质粘附性降低，倾向于保持圆形。
We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded.
鉴定特异性识别角膜和骨骼硫酸角质素的单克隆抗体。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Caterson,B;Christner,JE;Baker,JR
通讯作者：
Baker,JR
硫酸软骨素蛋白多糖生物合成的免疫荧光研究：单克隆抗体的使用。
DOI：
10.1016/s0174-173x(84)80025-4
发表时间：
1984
期刊：
Collagen and related research
影响因子：
--
作者：
Vertel,BM;Barkman,LL
通讯作者：
Barkman,LL