Application of lipid extracts from Solidago canadensis to phytomonitoring of PCB126 in transgenic Arabidopsis plants.
Application of lipid extracts from Solidago canadensis to phytomonitoring of PCB126 in transgenic Arabidopsis plants.
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DOI：
10.1016/j.scitotenv.2014.01.090
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Sayuri Shimazu;M. Ohta;H. Ashida
中科院分区：
文献类型：
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作者：
Sayuri Shimazu;M. Ohta;H. Ashida
本研究的目的是阐明加拿大一枝黄花脂质提取物对携带重组豚鼠 (g) 芳烃受体 (AhR) 介导的 β-葡萄糖醛酸酶 (GUS) 报告基因表达系统的转基因拟南芥植物 XgD2V11-6 中多氯联苯 (PCB) 126 的植物监测的影响。从 S 中制备脂质提取物。加拿大人通过硅胶柱色谱法将其分离成简单脂质、糖脂和磷脂级分。在糖脂部分中发现了硬脂基葡萄糖苷 (SG)、单半乳糖基二酰基甘油 (MGDG)、二半乳糖基二酰基甘油 (DGDG) 和葡萄糖基神经酰胺。当用糖脂级分与PCB126一起处理转基因拟南芥植物时，整个植物中PCB126诱导的GUS活性显着增加。此外，S.源自加拿大的 SG、MGDG 和 DGDG 也显着增加了 PCB126 诱导的 GUS 活性。这些结果表明糖脂在S中。 canadensis 增强了该监测测定的灵敏度。
The aim of this study is to elucidate the effect of lipid extracts fromSolidago canadensisfor phytomonitoring of polychlorinated biphenyl (PCB) 126 in the transgenicArabidopsisplant XgD2V11-6 carrying the recombinant guinea pig (g) aryl hydrocarbon receptor (AhR)-mediated β-glucuronidase (GUS) reporter gene expression system. A lipid extract was prepared fromS. canadensisand separated into simple lipid, glycolipid, and phospholipid fractions by silica gel column chromatography. Sterylglucoside (SG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and glucosyl ceramide were found in the glycolipid fraction. When the transgenicArabidopsisplants were treated with the glycolipid fraction together with PCB126, PCB126-induced GUS activity significantly increased in the whole plant. Moreover,S. canadensis-derived SG, MGDG, and DGDG also significantly increased PCB126-induced GUS activity. These results indicated that glycolipids inS. canadensisenhanced the sensitivity of this monitoring assay.