Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.

Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.
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DOI:
10.1039/c0cc01707h
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发表时间:
2011-01-07
期刊:
Chemical communications (Cambridge, England)
影响因子:
--
通讯作者:
Ying L
Ying L
中科院分区:
其他
文献类型:
--
作者:
Lenn T;Gkekas CN;Bernard L;Engl C;Jovanovic G;Buck M;Ying L

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We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.
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