Measurement of the microdistribution of strontium and lead in bone via benchtop monochromatic microbeam X-ray fluorescence with a low power source
Measurement of the microdistribution of strontium and lead in bone via benchtop monochromatic microbeam X-ray fluorescence with a low power source
复制标题
DOI：
10.1039/b820067j
复制
发表时间：
2009-01-01
影响因子：
3.4
通讯作者：
Parsons, Patrick J.
中科院分区：
文献类型：
--
作者：
Bellis, David J.;Li, Danhong;Parsons, Patrick J.
测定微量元素在骨中的微量分布在低浓度下，以前已经进行了质子诱导X射线发射（PIXE），高能同步辐射源X射线荧光（XRF）和激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）。目前有几种具有微米级分辨率的商用台式XRF系统。虽然提供了方便的、非破坏性的映射能力，但它们似乎缺乏检测生物组织（如骨）中微量元素所需的灵敏度。我们研究了一个原型台式XRF系统的应用，用于测量锶和铅在骨中的生理水平。几个感兴趣的元素，包括Sr和Pb的检测实现了与实验设置的基础上聚焦单色微束X射线荧光（M μ-XRF）仪器与低功率源（45 W钼管）耦合到双弯曲晶体（DCC）光学。一个横截面的骨约5毫米× 8毫米大小的映射与80 μ m的分辨率显示不均匀分布的Sr和Pb。结果表明，M μ-XRF与DCC联用是测定骨中微量元素空间分布的有效方法。
Determination of the microdistribution of trace elements in bone at low concentrations has previously been performed with proton induced X-ray emission (PIXE), high-energy synchrotron source X-ray fluorescence (XRF) and laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS). Several commercial benchtop XRF systems with micrometer-scale resolution are currently available. While providing convenient, non-destructive mapping capability, they appear to lack the sensitivity required for detection of trace elements in biological tissues such as bone. We investigated the application of a prototype benchtop XRF system for the measurement of strontium and lead at physiological levels in bone. Detection of several elements of interest, including Sr and Pb was achieved with an experimental set up based on focused monochromatic microbeam X-ray fluorescence (M mu-XRF) instrumentation with a low power source (45 W molybdenum tube) coupled to doubly curved crystal (DCC) optics. A cross-section of bone about 5 mm x 8 mm size was mapped with 80-mu m resolution showing heterogeneous distribution of Sr and Pb. The data showed that M mu-XRF coupled to DCC is powerful method for measurement of the spatial distribution of trace elements in bone.