Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked.
Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked.
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使用 xylE 融合来证明 lsi-1（一种淋病奈瑟菌脂寡糖生物合成基因）和 lsi-3 不存在转录关联。
DOI：
10.1128/jb.176.11.3428-3432.1994
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发表时间：
1994
影响因子：
3.2
通讯作者：
Stein,DC
中科院分区：
文献类型：
--
作者：
Danaher,RJ;Petricoin3rd,EF;Stein,DC
淋病奈瑟菌脂寡糖生物合成基因 lsi-1 前面有一个 281 bp 非蛋白质编码序列 lsi-3，其中包含两对反向重复序列。生成淋球菌染色体 lsi-xylE 基因融合体以测量二级结构对转录衰减的影响。获得的数据表明lsi-3和lsi-1没有转录连接，因此lsi-3不参与lsi-1的调节。
The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.