Indole contributes to tetracycline resistance via the outer membrane protein OmpN in Vibrio splendidus
Indole contributes to tetracycline resistance via the outer membrane protein OmpN in Vibrio splendidus
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吲哚通过灿烂弧菌的外膜蛋白 OmpN 促进四环素耐药性
DOI：
10.1007/s11274-020-02813-6
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发表时间：
2020-02
影响因子：
4.1
通讯作者：
Weiwei Zhang
中科院分区：
文献类型：
--
作者：
Shanshan Zhang;Yina Shao;Xuelin Zhao;Chenghua Li;Ming Guo;Zhimeng Lv;Weiwei Zhang
吲哚作为一种跨种、跨界的信号分子，因其对细菌和宿主的多种生理效应而受到广泛关注。本研究中，吲哚增加了灿烂弧菌的四环素抗性。四环素的最小抑菌浓度为10 μg/mL，当四环素浓度为20 μg/mL时，灿烂弧菌的OD 600降低了94.5%，而当四环素浓度为20 μ g/mL时，吲哚浓度为125 μM时，灿烂弧菌的OD 600在不同时间点分别比四环素浓度为20 μg/mL时高出10倍和4.5倍。在OD 600约为2.0（吲哚水平较高）的培养液中，对10 μg/mL四环素的抗性细胞比例比OD 600为0.5的培养液高600倍，表明吲哚水平与灿烂弧菌的四环素抗性相关。此外，一个差异表达的蛋白质，这是确定为外膜孔蛋白OmpN通过SDS-PAGE结合MALDI-TOF/TOF MS，上调。因此，与对照样品相比，在四环素或吲哚存在下以及同时在吲哚和四环素存在下ompN基因的表达分别上调1.8倍、2.54倍和6.01倍。综合以上结果表明，吲哚增强了灿烂弧菌对四环素的抗性，这种抗性可能与外膜孔蛋白OmpN的表达上调有关。
As an interspecies and interkingdom signaling molecule, indole has recently received attention for its diverse effects on the physiology of both bacteria and hosts. In this study, indole increased the tetracycline resistance of Vibrio splendidus. The minimal inhibitory concentration of tetracycline was 10 μg/mL, and the OD600of V. splendidus decreased by 94.5% in the presence of 20 μg/mL tetracycline; however, the OD600of V. splendidus with a mixture of 20 μg/mL tetracycline and 125 μM indole was 10- or 4.5-fold higher than that with only 20 μg/mL tetracycline at different time points. The percentage of cells resistant to 10 μg/mL tetracycline was 600-fold higher in the culture with an OD600of approximately 2.0 (higher level of indole) than that in the culture with an OD600of 0.5, which also meant that the level of indole was correlated to the tetracycline resistance of V. splendidus. Furthermore, one differentially expressed protein, which was identified as the outer membrane porin OmpN using SDS-PAGE combined with MALDI-TOF/TOF MS, was upregulated. Consequently, the expression of the ompN gene in the presence of either tetracycline or indole and simultaneously in the presence of indole and tetracycline was upregulated by 1.8-, 2.54-, and 6.01-fold, respectively, compared to the control samples. The combined results demonstrated that indole enhanced the tetracycline resistance of V. splendidus, and this resistance was probably due to upregulation of the outer membrane porin OmpN.
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影响因子：
5.7
作者：
Lee J;Attila C;Cirillo SL;Cirillo JD;Wood TK
通讯作者：
Wood TK
信号介导的细菌持久形成。
影响因子：
14.8
作者：
Vega, Nicole M.;Allison, Kyle R.;Khalil, Ahmad S.;Collins, James J.
通讯作者：
Collins, James J.
DOI：
10.1016/j.bbapap.2008.11.005
发表时间：
2009-05
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
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通讯作者：
Delcour AH
影响因子：
2.5
作者：
H. L. Macpherson;Ø. Bergh;T. Birkbeck
通讯作者：
H. L. Macpherson;Ø. Bergh;T. Birkbeck
DOI：
10.2139/ssrn.3114224
发表时间：
2018-01
期刊：
NBER Working Paper Series
影响因子：
--
作者：
Gary B. Gorton;Toomas Laarits
通讯作者：
Gary B. Gorton;Toomas Laarits