Hypoxia promotes growth of stem cells in dental follicle cell populations.
Hypoxia promotes growth of stem cells in dental follicle cell populations.
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DOI：
10.4236/jbise.2011.46057
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Yao S
中科院分区：
文献类型：
--
作者：
Dai Y;He H;Wise GE;Yao S
成体干细胞(ASC)广泛存在于多种组织中，由于其具有分化能力，具有很大的治疗潜力。然而，ASC只占组织的一小部分。为了将ASC用于治疗目的，大量获得相对纯净的干细胞是很重要的。目前的干细胞纯化方法主要是基于标记物依赖的细胞分选技术，存在各种技术困难。在这项研究中，我们试图开发新的条件，以利于牙囊干细胞(DFSC)的生长，从而使所产生的细胞群在干细胞中得到丰富。具体地说，含有干细胞的异质性牙囊细胞群(H-DFC)和同源非干细胞牙囊细胞群在1%或5%的低氧条件下培养。在低氧条件下，只有异种种群才能促进其增殖，而同源DFC不改变其增殖率。此外，当异种细胞群中的细胞进行分化时，它们似乎比在正常气氛(常氧条件)下生长的对照组具有更高的成脂和成骨能力。这些经过低氧处理的细胞也表达更高水平的某些干细胞标记物。总而言之，这些数据表明，通过在氧气减少的条件下培养不同种类的细胞群，干细胞得到了丰富。
Adult stem cells (ASC) have been found in many tissues and are of great therapeutic potential due to their capability of differentiation. However, ASC comprise only a small fraction of the tissues. In order to use ASC for therapeutic purposes, it is important to obtain relatively pure stem cells in large quantities. Current methods for stem cell purification are mainly based on marker-dependent cell sorting techniques, which have various technical difficulties. In this study, we have attempted to develop novel conditions to favor the growth of the dental follicle stem cells (DFSC) such that the resultant cell populations are enriched in stem cells. Specifically, a heterogeneous dental follicle cell (H-DFC) population containing stem cells and homogenous non-stem cell dental follicle cell population were cultured at 1% or 5% hypoxic conditions. Only the heterogeneous population could increase proliferation in the hypoxic condition whereas the homogenous DFC did not change their proliferation rate. In addition, when the resultant cells from the heterogonous population were subjected to differentiation, they appeared to have a higher capacity of adipogenesis and osteogenesis as compared to the controls grown in the normal atmosphere (normoxic condition). These hypoxia-treated cells also express higher levels of some stem cell markers. Together, these data suggest that stem cells are enriched by culturing the heterogeneous cell populations in a reduced O2 condition.