Fixatives and fixation: a review.
Fixatives and fixation: a review.
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DOI：
10.1007/bf01003278
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发表时间：
1969-05-01
期刊：
影响因子：
--
通讯作者：
Hopwood, D
中科院分区：
文献类型：
--
作者：
Hopwood, D
结论在本报告所述期间，已推出了各种新的固定剂。其中最重要的是戊二醛，它已被广泛使用。固色过程在化学和物理方面得到了更好的理解，这在很大程度上要归功于生化和制革的发展。随着电子显微镜对大分子分辨率的提高，这种理解将变得越来越重要。然后，所看到的图像将能够根据大分子的生物化学知识进行解释，从而弥合形态和生物化学之间的鸿沟。一些固定剂具有二次用途，因为它们可以展示化学物质。另一些显然是选择性地破坏某些生物活动。越来越多的人注意到在制备过程中生物材料中的物质损失。这类研究的重要性怎么强调都不为过。没有一种固定剂对所有情况都是理想的。通过比较几种固定剂获得的结果，并评估加工过程中物质的损失，人工制品被描述为恒定特征的可能性要小得多。
ConclusionsDuring the period under review, various new fixatives have been introduced. The most important of these is glutaraldehyde which has become very widely used. The processes involved in fixation are becoming better understood in chemical and physical terms, largely due to developments in biochemistry and tanning. This understanding will become increasingly important as the resolution of macromolecules by the electron microscope increases. The images seen will then be able to be interpreted in the light of biochemical knowledge of the macromolecules and thus the gap between morphology and biochemistry will be bridged.Some of the fixatives have secondary uses in that they may demonstrate chemical substances. Others apparently destroy certain biological activities selectively.Increasing attention is being paid to the loss of substances from biological material during preparation. The importance of such studies cannot be overemphasized.No one fixative is ideal for all situations. By comparing the results obtained by a number of fixatives, and assessing the loss of substances during processing, it is far less likely that artefacts will be described as constant features.