Oocyte apoptosis during the transition from ovary-like tissue to testes during sex differentiation of juvenile zebrafish.
Oocyte apoptosis during the transition from ovary-like tissue to testes during sex differentiation of juvenile zebrafish.
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发表时间：
2002-03
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通讯作者：
D. Uchida;M. Yamashita;T. Kitano;T. Iguchi
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作者：
D. Uchida;M. Yamashita;T. Kitano;T. Iguchi
大量的凋亡早期双线期卵母细胞的过渡过程中观察到卵巢样未分化的性腺组织睾丸在性别分化的斑马鱼（Danio rerio）的假定男性。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）阳性凋亡的卵母细胞在性腺的推定男性的百分比约为8至12倍，高于遗传的所有女性。到孵化后29天，所有的卵母细胞已经从推定的男性性腺消失。在这些雄性中，我们还观察到孵化后23至35天卵巢腔中的体细胞凋亡。因此，性分化过程中细胞凋亡引起的卵母细胞消失和卵巢腔分解是雄性特有的事件。在遗传全雌性中，在孵化后15-19天的未分化性腺中发现了一部分早期双线期卵母细胞的凋亡，这可能是卵巢发育过程中卵母细胞程序性丢失的结果。这些结果表明，卵母细胞凋亡是斑马鱼睾丸和卵巢分化的机制。
Large numbers of apoptotic early diplotene oocytes were observed during the transition from ovary-like undifferentiated gonadal tissue to testes during sex differentiation in presumptive males of the zebrafish (Danio rerio). The percentage of terminal-deoxynucleotidyl-transferase-mediated dUTP nick-end labelling (TUNEL)-positive apoptotic oocytes in the gonads of presumptive males was approximately eight- to 12-fold higher than in genetic all-females. By 29 days post-hatching, all oocytes had disappeared from the gonads of presumptive males. In these males, we also observed apoptotic somatic cells in the ovarian cavity between 23 and 35 days post-hatching. Therefore, the disappearance of oocytes and the decomposition of the ovarian cavity caused by apoptosis during sex differentiation were male-specific events. In genetic all-females, apoptosis in a proportion of early diplotene oocytes was found in the undifferentiated gonads at 15-19 days post-hatching, probably as a result of programmed oocyte loss during ovarian development. These findings suggest that oocyte apoptosis is the mechanism of testicular and ovarian differentiation in zebrafish.