Genome sequence of the cat pathogen, Chlamydophila felis
Genome sequence of the cat pathogen, Chlamydophila felis
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DOI：
10.1093/dnares/dsi027
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发表时间：
2006-02-28
期刊：
影响因子：
4.1
通讯作者：
Shirai, Mutsunori
中科院分区：
文献类型：
--
作者：
Azuma, Yoshinao;Hirakawa, Hideki;Shirai, Mutsunori
猫肺炎衣原体(Chlamydia Psittali猫科动物肺炎病原体)是猫肺炎和结膜炎的世界性传播病原体。在此，我们测定了日本猫毛虫Fe/C-56株的全基因组DNA序列，以了解该病原菌引起疾病的机制。其基因组由1 166 239bp的环状染色体和7552bp的环状质粒组成，编码1005个蛋白质编码基因。通过与其他衣原体的基因含量比较，发现衣原体共有795个基因，47个基因是衣原体所特有的。对共同基因的系统发育分析表明，大多数同源基因集表现出相似的分化模式，但14个猫砂基因积累了更多的突变，这意味着这些基因可能参与了对猫砂特有生态位的进化适应。基因分布和同源分析表明，两个不同的区域，即可塑性区域和频繁基因易位区(Fgrs)，可能在衣原体基因组进化中发挥着重要但不同的作用。费氏衣原体基因组DNA序列为理解疾病和阐明衣原体进化提供了信息。
Chlamydophila felis (Chlamydia psittaci feline pneumonitis agent) is a worldwide spread pathogen for pneumonia and conjunctivitis in cats. Herein, we determined the entire genomic DNA sequence of the Japanese C. felis strain Fe/C-56 to understand the mechanism of diseases caused by this pathogen. The C. felis genome is composed of a circular 1 166 239 bp chromosome encoding 1005 protein-coding genes and a 7552 bp circular plasmid. Comparison of C. felis gene contents with other Chlamydia species shows that 795 genes are common in the family Chlamydiaceae species and 47 genes are specific to C. felis. Phylogenetic analysis of the common genes reveals that most of the orthologue sets exhibit a similar divergent pattern but 14 C. felis genes accumulate more mutations, implicating that these genes may be involved in the evolutional adaptation to the C. felis-specific niche. Gene distribution and orthologue analyses reveal that two distinctive regions, i.e. the plasticity zone and frequently gene-translocated regions (FGRs), may play important but different roles for chlamydial genome evolution. The genomic DNA sequence of C. felis provides information for comprehension of diseases and elucidation of the chlamydial evolution.