GABA-IMMUNOREACTIVE NEURONS IN THE RAT CEREBELLUM - A LIGHT AND ELECTRON-MICROSCOPE STUDY
GABA-IMMUNOREACTIVE NEURONS IN THE RAT CEREBELLUM - A LIGHT AND ELECTRON-MICROSCOPE STUDY
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DOI：
10.1002/cne.902510404
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发表时间：
1986-09-22
影响因子：
2.5
通讯作者：
HAMORI, J
中科院分区：
文献类型：
--
作者：
GABBOTT, PLA;SOMOGYI, J;HAMORI, J
抗γ抗体产生的抗γ的抗体- 用戊二醛偶联牛血清白蛋白的氨基丁酸（GABA）（Hodgson等人：J. Histochem.细胞化学33：229-239，′ ′ 85）用于免疫细胞化学地定位成年大鼠小脑皮质中假定的GABA能神经元元件。采用未标记的抗体酶法与前和postbedding免疫细胞化学程序，以下细胞结构被观察到GABA免疫阳性的光镜和电镜：星状，篮状和高尔基神经元的胞体，树突和轴突过程（包括轴突终端）。在免疫阳性的神经元胞体和树突，反应产物被发现与所有细胞内的细胞器和突触连接的突触后密度。在线粒体外膜、微管和神经丝周围以及突触前轴突终末的突触囊泡周围也可见特异性GABA样免疫反应。在预包埋程序与1：1，000和1：2，000之间的抗血清稀释度，浦肯野细胞的胞体和树突GABA免疫阴性，而在1：500的抗血清稀释度的浦肯野细胞的胞体是轻度GABA免疫反应。浦肯野细胞轴突终末在下和上神经节丛和小脑深核始终强烈免疫阳性。神经胶质细胞总是GABA免疫阴性，树突，轴突（平行纤维），和颗粒细胞的胞体。苔藓纤维和攀缘纤维传入也呈免疫阴性。用这种抗抑制性神经递质GABA的抗血清获得的免疫反应性模式被发现与GABA和GABA合成酶谷氨酸脱羧酶（GAD）的免疫细胞化学分布非常相似，如先前在其他免疫细胞化学研究中所报道的（Oertel等和Wu等：Cytochemical Methods in Neuroanatomy.纽约：A. R.利斯。82; Seguela等人：Neuroscience 16：865-874，85; Mugnaini和Oertel：GABA和CNS中的神经肽。化学神经解剖学手册，第4卷，第一部分。Amsterdam：Elsevier，'' 85）：.
An antibody raised against .gamma. - amino-butyric acid (GABA) coupled to bovine serum albumin with glutaraldehyde (Hodgson et al: J. Histochem. Cytochem. 33:229-239, ''85) was used to localise immunocytochemically the presumptive GABAergic neuronal elements in the cerebellar cortex of the adult rat. Employing the unlabelled antibody enzyme method with pre- and postembedding immunocytochemical procedures, the following cellular structutures were observed to be GABA-immunopositive in both the light and electron microscopes: the somata, dendrites, and axonal processes (including axon terminals) of stellate, basket, and Golgi neurons. In immunopositive neuronal somata and dendrites, the reaction product was found to be associated with all intracellular organelles and with the postsynaptic densities of synaptic junctions. Specific GABA-like immunoreactivity was also seen around outer mitochondrial membranes, microtubules, and neurofilaments, and coating synaptic vesicles in presynaptic axon terminals. In the pre-embedding procedure with dilutions of the antiserum between 1:1,000 and 1:2,000, the perikarya and dendrites of Purkinje cells were GABA-immunonegative, whereas at an antiserum dilution of 1:500 the somata of Purkinje cells were mildly GABA-immunoreactive. Purkinje cell axon terminals in the infra- and supraganglionic plexuses and in the deep cerebellar nuclei were always strongly immunopositive. Neuroglia were invariably GABA-immunonegative, as were the dendrites, axons (parallel fibres), and somata of granule cells. Mossy fibre and climbing fibre afferents were also immunonegative. The pattern of immunoreactivity obtained with this antiserum directed against the inhibitory neurotransmitter GABA was found to resemble closely the immunocytochemical distribution of GABA and of the GABA-synthesizing enzyme glutamic acid decarboxylase (GAD) as reported previously in other immunocytochemical investigations (Oertel et al. and Wu et al: Cytochemical Methods in Neuroanatomy. New York: A. R. Liss. ''82; Seguela et al: Neuroscience 16:865-874, ''85; Mugnaini and Oertel: GABA and Neuropeptides in the CNS. Handbook of Chemical Neuroanatomy, Vol. 4, Part I. Amsterdam: Elsevier, ''85):.