An environmentally-relevant mixture of organochlorines and its vehicle control, dimethylsulfoxide, induce ultrastructural alterations in porcine oocytes
An environmentally-relevant mixture of organochlorines and its vehicle control, dimethylsulfoxide, induce ultrastructural alterations in porcine oocytes
复制标题
DOI：
10.1002/mrd.20365
复制
发表时间：
2006-01-01
影响因子：
2.5
通讯作者：
Maddox-Hyttel, P
中科院分区：
文献类型：
--
作者：
Campagna, C;Bailey, JL;Maddox-Hyttel, P
有机氯化学品在环境中积累，特别是在北极地区，对野生动物和人类健康构成潜在的发育危害。尽管它们的一些有害影响已被认识到，但它们在目标细胞内的作用机制需要更好地了解。这项研究旨在验证一种与环境相关的有机氯混合物在猪模型中改变卵母细胞超微结构的假设。未成熟的卵丘-卵母细胞复合体(COCs)、未经处理的部分培养(18h)COCs以及在培养过程中暴露于有机氯混合物或其载体(0.1%二甲基亚砜；DMSO)的COCs进行光镜和透射电子显微镜观察。有机氯可引起COCs的主要超微结构改变：脂滴密度降低，光滑内质网(SER)体积增大，SER与线粒体、脂滴和囊泡之间的相互作用增强。我们认为，这些超微结构的变化促进了产生代谢酶所需的能量形成。其他超微结构变化可能反映了一定程度的有机氯毒性：缝隙连接减少和皮质颗粒电子密度降低。出乎意料的是，DMSO对照处理也引起了类似的超微结构变化，但程度低于有机氯混合物。本研究首次证实了环境污染物对哺乳动物卵母细胞超微结构的影响。
Organochlorine chemicals accumulate in the environment, particularly in the Arctic, and constitute potential developmental hazards to wildlife and human health. Although some of their harmful effects are recognized, their mechanisms of action within the target cells need to be better understood. This study was designed to test the hypothesis that an environmentally-relevant organochlorine mixture alters oocyte ultrastructure in the porcine model. Immature cumulus-oocyte complexes (COCs), partially cultured (18 hr) COCs without treatment or exposed to the organochlorine mixture or its vehicle (0.1% dimethysulfoxide; DMSO) during culture were processed for light and transmission electronic microscopy (TEM). The organochlorines induced major ultrastructural changes in the COCs: decreased density of the lipid droplets, increased smooth endoplasmic reticulum (SER) volume and increased interactions among SER, mitochondria, lipid droplets and vesicles. We suggest that these ultrastructural changes facilitate energy formation necessary to produce metabolizing enzymes. Other ultrastructural changes may reflect some degree of organochlorine toxicity: fewer gap junctions and decreased electron density of the cortical granules. Unexpectedly, the DMSO control treatment also induced similar ultrastructural changes, but to a lesser degree than the organochlorine mixture. This study is the first to demonstrate the effect of environmental contaminants on mammalian oocyte ultrastructure.