Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1)
Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1)
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DOI：
10.1093/oxfordjournals.jbchem.a003109
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发表时间：
2002-03-01
影响因子：
2.7
通讯作者：
Hayashi, K
中科院分区：
文献类型：
--
作者：
Fukuda, S;Yamasaki, Y;Hayashi, K
c - myc内含子结合蛋白1（MIBP1）是一种巨大的锌指蛋白，包含2437个氨基酸，属于主要组织相容性复合体（MHC）结合蛋白（MBP）家族。MIBP1被认为是一种参与多种生物学功能的转录因子。我们在此表明，MIBP1抑制来自主要启动子P2的c - myc转录。通过酵母双杂交系统筛选发现，MIBP1蛋白与Ski相互作用蛋白（SKIP）相互作用。体外下拉实验和体内免疫共沉淀实验证实了这种相互作用。发现MIBP1的酸性区域是与SKIP的N端一半相互作用的位点。利用发育中的大鼠胚胎进行的原位杂交分析显示，MIBP1 mRNA在有丝分裂后的神经元中高度表达，但在未成熟的神经上皮中的表达较低。MIBP1在成年大鼠大脑中的表达也主要在神经元细胞中，这表明MIBP1参与成熟神经元细胞的生理过程。
The c-myc intron binding protein 1 (MIBP1) is a gigantic zinc finger protein comprising 2,437 amino acids and belonging to the MHC binding protein (MBP) family. MIBP1 is suggested to be a transcription factor involved in various biological functions. We show here that MIBP1 represses c-myc transcription from the major promoter, P2. Screening by the yeast two-hybrid system revealed that the MIBP1 protein interacts with the Ski-interacting protein (SKIP). In vitro pull-down assays and in vivo co-immunoprecipitation experiments confirmed this interaction. The acidic region of MIBP1 was found to be the site of interaction with the N-terminal half of SKIP. In situ hybridization analysis using developing rat embryos revealed that the MIBP1 mRNA is highly expressed in post-mitotic neurons, but the expression in immature neuroepithelium is low. The expression of MIBP1 in adult rat brain is also predominantly in neuronal cells, indicating that MIBP1 is involved in the physiology of mature neuronal cells.