In situ visualization of paclitaxel distribution and release by coherent anti-stokes Raman scattering microscopy
In situ visualization of paclitaxel distribution and release by coherent anti-stokes Raman scattering microscopy
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DOI：
10.1021/ac061218s
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发表时间：
2006-12-01
影响因子：
7.4
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
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作者：
Kang, Eunah;Wang, Haifeng;Cheng, Ji-Xin
可视化药物分子的三维分布和释放过程中的后续变化对于理解药物递送机制以及设计定制的释放曲线至关重要。本研究利用相干反斯托克斯拉曼散射（汽车）成像检查紫杉醇分布在各种聚合物膜的横向分辨率为0.3 μ m和深度分辨率为0.9 μ m。CH伸缩振动和指纹区域中的拉曼谱带用于区分紫杉醇和聚合物。通过对溶解在N，N-二甲基甲酰胺溶液中的紫杉醇分子成像，检测灵敏度测量为29 mM。以1帧/秒的采集速度监测紫杉醇从聚合物基质的释放。我们的研究结果表明，汽车显微镜可以有效地用于在原位成像的天然药物分子的输送系统。
Visualization of three-dimensional distribution of drug molecules and subsequent changes during the release process is critical for understanding drug delivery mechanisms as well as designing tailor-made release profiles. This study utilized coherent anti-Stokes Raman scattering ( CARS) imaging to examine paclitaxel distribution in various polymer films with lateral resolution of 0.3 mu m and depth resolution of 0.9 mu m. Raman bands in the CH stretch vibration and fingerprint regions were used to distinguish paclitaxel from the polymers. The detection sensitivity was measured to be 29 mM by imaging paclitaxel molecules dissolved in N,N-dimethylformamide solution. Release of paclitaxel from a polymer matrix was monitored at an acquisition speed of 1 frame/s. Our results show that CARS microscopy can be used effectively for in situ imaging of native drug molecules in a delivery system.