A Quantitative Proteomics Study of Early Heat-Regulated Proteins by Two-Dimensional Difference Gel Electrophoresis Identified OsUBP21 as a Negative Regulator of Heat Stress Responses in Rice
A Quantitative Proteomics Study of Early Heat-Regulated Proteins by Two-Dimensional Difference Gel Electrophoresis Identified OsUBP21 as a Negative Regulator of Heat Stress Responses in Rice
复制标题
通过二维差异凝胶电泳对早期热调节蛋白进行定量蛋白质组学研究，确定 OsUBP21 是水稻热应激反应的负调节因子
DOI：
10.1002/pmic.201900153
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发表时间：
2019
期刊：
影响因子：
3.4
通讯作者：
Zhang Baowen
中科院分区：
文献类型：
--
作者：
Zhou Hangfan;Wang Xiaolong;Huo Chenmin;Wang Hui;An Zhichao;Sun Daye;Liu Jingze;Tang Wenqiang;Zhang Baowen
为了了解早期热激（HS）调节的细胞反应影响水稻对高温环境的耐受性，采用二维差异凝胶电泳（2D-DIGE）技术对早期HS调节的蛋白质组进行了研究。鉴定了在HS处理1和5分钟后显示丰度变化的多种蛋白质。在鉴定的早期HS调节蛋白中，发现泛素特异性蛋白酶OsUBP 21和它的泛素同源物AtUBP 13的丰度通过HS处理5分钟而上调。此外，敲低或敲除OsUBP 21或AtUBP 13的表达增加了水稻和拟南芥植物对HS胁迫的耐受性，表明这些泛素特异性蛋白酶在调节植物HS响应中的功能在单子叶植物和双子叶植物之间是保守的。2D-DIGE结果显示，HS处理30 min后，野生型和dubp 21突变体中有一组蛋白质表达差异。在这些蛋白质中，发现11个与OsUBP 21直接相互作用，因此，它们可能是OsUBP 21的靶标。未来对这些OsUBP 21相互作用蛋白在植物HS反应中的作用的分析将有助于揭示HS诱导的水稻蛋白泛素化/去泛素化调控的细胞反应。
To understand the early heat shock (HS)‐regulated cellular responses that influence the tolerance of rice plant to high environmental temperatures, two‐dimensional difference gel electrophoresis (2D‐DIGE) is performed to explore the early HS‐regulated proteome. Multiple proteins that show abundance changes after 1 and 5 min of HS treatment are identified. Of the early HS‐regulated proteins identified, the abundance of a ubiquitin‐specific protease, OsUBP21, and itsArabidopsishomolog, AtUBP13, is found to be upregulated by 5 min of HS treatment. Further, knocking the expression ofOsUBP21orAtUBP13down or out increases the tolerance of rice andArabidopsisplants to HS stress, suggesting that the function of these ubiquitin‐specific proteases in regulating plant HS responses is conserved between monocots and dicots. 2D‐DIGE showed a group of proteins are differentially regulated in wild‐type andubp21mutant after 30 min of HS treatment. Among these proteins, 11 are found to interact directly with OsUBP21; thus, they may be targets of OsUBP21. Future analyses of the roles of these OsUBP21‐interacting proteins in plant HS responses will help reveal the protein ubiquitination/deubiquitination‐regulated cellular responses induced by HS in rice.