Transcriptional activation of the promoter of human cytomegalovirus immediate early gene (CMV-1E) by the hepatitis B viral X protein (HBx) through the NF-κB site
Transcriptional activation of the promoter of human cytomegalovirus immediate early gene (CMV-1E) by the hepatitis B viral X protein (HBx) through the NF-κB site
复制标题
DOI：
10.1016/s0168-1702(01)00445-2
复制
发表时间：
2002-03-20
期刊：
影响因子：
5
通讯作者：
Rho, HM
中科院分区：
文献类型：
--
作者：
Assogba, BD;Choi, BH;Rho, HM
人的潜伏巨细胞病毒(CMV)被另一种病毒感染重新激活，可能会引起病毒相关症状。基于这一假设，我们研究了HBx对CMV-IE激活的影响，CMV-IE也被称为反式激活因子和潜在的癌基因。HBx在人肝细胞株HepG2和肺成纤维细胞MRC-5中分别反式激活CMV-IE启动子4倍和18倍。将HBx与CMV-IE启动子中的每个转录因子共转染后发现，只有NF-kappaB能协同激活启动子，激活倍数可达14倍。序列缺失分析和点突变分析表明，第三个核因子-kappaB位点(NT-267~-258)和第二个核因子-kappaB位点(NT-162~-153)分别是反式激活的主要负责位点和次要作用位点。这些结果表明，先前感染CMV的细胞的乙肝病毒感染可能会影响人体内潜伏的巨细胞病毒的重新激活，从而引起病毒相关症状。(C)2002 Elsevier Science B.V.保留所有权利。
The reactivation of latent cytomegalovirus (CMV) in a human by another viral infection may induce virus-related symptoms. Based on this presumption, we investigated the effect of HBx on the activation of the CMV-IE, which is also known as a transactivator and potential oncogene. The HBx transactivated the CMV-IE promoter by up to 4- and 18-fold factors in human liver HepG2 and lung fibroblast MRC-5 cells, respectively. Cotransfection of HBx with each transcription factor presented in the CMV-IE promoter showed that only NF-kappaB synergistically activated the promoter by up to a 14-fold factor. Serial deletion assays and point mutation analysis showed that the third NF-kappaB site (nt - 267 to - 258) and the second one (nt - 162 to - 153) appeared as the major responsible site and minor one, respectively, for the transactivation. These results suggest the possibility that the HBV infection of a cell previously infected by CMV would exert influence on the reactivation of the latent cytomegalovirus in a human to induce virus-related symptoms. (C) 2002 Elsevier Science B.V. All rights reserved.