Unrestrained plethysmography is an unreliable measure of airway responsiveness in BALB/c and C57BL/6 mice
Unrestrained plethysmography is an unreliable measure of airway responsiveness in BALB/c and C57BL/6 mice
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DOI：
10.1152/japplphysiol.00821.2003
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发表时间：
2004-07-01
影响因子：
3.3
通讯作者：
Irvin, CG
中科院分区：
文献类型：
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作者：
Adler, A;Cieslewicz, G;Irvin, CG
Hamelmann等人（Am J Respir Crit Care Med 156：766-775，1997）描述的技术已经得到了大量利用，其中通过无限制的体积描记法（IT）非侵入性地测量与气道反应性相关的参数增强暂停（Penh）。调查这项技术，我们试图回答这些问题：1）如何在Penh的变化与传统的体积描记和肺机械参数的变化相比？2)UP参数如何在两种不同的小鼠品系中执行？将清醒免疫的和对照BALB/c（n = 16）和C57 BL/6（n = 14）小鼠置于UP室中并暴露于一定剂量的acrosolized乙酰甲胆碱，同时在每个浓度下测量以下参数：吸气时间（TI）、呼气时间（TE）、总时间（Ttot）、Ti/Ttot、吸气峰压、呼气峰压、VT、Penh、潮气量（VT）、VT/Ti，VT/TF和VT/Ttot。第二天，在相同的动物中侵入性地测量肺阻力（RL）和顺应性（CL）。对于BALB/c，与RL具有最高相关系数幅度的参数是（按顺序）1）CL，2）CL和Penh，3）呼吸频率参数（TE、Ttot、TH和4）与VT（吸气压、呼气压）相关的参数。流量参数（VT/Ttot、VT/TE、VT/TI）和占空比参数（Tifrtot）相关性不显著。这种排序在C57 BL/6小鼠中显著不同，其中具有最大相关性的参数是1）CL，2）呼吸频率参数和3）流量参数。PENh、VT和占空比参数的相关性不显著。这些数据表明，Penh是有问题的，因为它是品系特异性的;它在BALB/c和C57 BL/6小鼠中的表现非常不同。我们认为，UP参数主要起源于呼吸过程的反射控制的一部分，而不是在肺力学，并得出结论，这是不合适的使用UP参数一般，和Penh具体，作为替代变量的侵入性机械指标，如RL。
There has been significant utilization of the technique described by Hamelmann et al. (Am J Respir Crit Care Med 156: 766-775, 1997) in which a parameter, enhanced pause (Penh), related to airways responsiveness is noninvasively measured by unrestrained plethysmography (IT). Investigating this technique, we sought to answer these questions: 1) How do changes in Penh compare with changes in traditional plethysmographic and lung mechanical parameters? 2) How do UP parameters perform in two different mouse strains? Awake immunized and control BALB/c (n = 16) and C57BL/6 (n = 14) mice were placed in the UP chamber and exposed to doses of acrosolized methacholine while the following parameters were measured at each concentration: inspiratory time (TI), expiratory time (TE), total time (Ttot), Ti/Ttot, peak inspiratory pressure, peak expiratory pressure, Pause, Penh, tidal Volume (VT), VT/Ti, VT/TF, and VT/Ttot. The next day, lung resistance (RL) and compliance (CL) were invasively measured in the same animals. For the BALB/c, the parameters with the highest magnitude of correlation coefficient vs. RL are (in order) 1) CL, 2) Pause and Penh, 3) parameters of breathing frequency (TE, Ttot, TH, and 4) parameters related to VT (inspiratory pressure, expiratory pressure). Flow parameters (VT/Ttot, VT/TE, VT/TI) and duty cycle parameters (Tifrtot) had insignificant correlations. This ordering is significantly different in C57BL/6 mice, in which the parameters with the largest correlations are 1) CL, 2) parameters of breathing frequency, and 3) flow parameters. Pause, Penh, VT, and duty cycle parameters had insignificant correlations. These data show that Penh is problematic in the sense that it is strain specific; it behaves very differently in BALB/c and C57BL/6 mice. We suggest that UP parameters largely originate as part of reflex control of breathing processes, rather than in the lung mechanics and conclude that it is inappropriate to use UP parameters in general, and Penh specifically, as substitute variables for invasive mechanical indexes such as RL.