Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in an m/z Zone of Minimal Interference for Quantifiable, Multiplexed GC-MS Analyses.
Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in an m/z Zone of Minimal Interference for Quantifiable, Multiplexed GC-MS Analyses.
复制标题
电子电离诱导在干扰最小的 m/z 区内释放编码同位素报告离子，以进行可定量的多重 GC-MS 分析。
DOI：
10.1039/c3ay41124a
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Nantz,MichaelH
中科院分区：
文献类型：
--
作者：
Laulhé,Sébastien;Geers,TylerE;Shi,Xue;Zhang,Xiang;Nantz,MichaelH
我们描述了一种同位素编码策略，该策略能够同时对多个样品进行GC-MS分析，以进行底物鉴定和定量。该方法依赖于在m/z 31-37的最小干扰区（ZMI）中直接测量同位素乙基碳正离子作为质谱标签。样品醛和酮的混合物与同位素2-氨氧乙基丙酸酯反应，以说明该方法，确定的相对丰度的混合化合物的平均95%的准确度。ZMI报告离子检测还能够进行化学选择性底物分析和绝对定量，如使用生物来源的样品所证明的。
We describe an isotope coding strategy that enables simultaneous GC-MS analysis of multiple samples for substrate identification and quantification. The method relies on direct measurement of isotopic ethyl carbenium ions serving as mass spectral tags in a zone of minimal interference (ZMI) at m/z 31–37. Sample aldehyde and ketone mixtures were reacted with isotopic 2-aminooxyethyl propionates to illustrate the method, which determined the relative abundance of the mixed compounds with an average 95% accuracy. ZMI reporter ion detection also enables chemoselective substrate profiling and absolute quantification, as demonstrated using a biologically derived sample.