XC_0531 encodes a c-type cytochrome biogenesis protein and is required for pathogenesis in Xanthomonas campestris pv. campestris.
XC_0531 encodes a c-type cytochrome biogenesis protein and is required for pathogenesis in Xanthomonas campestris pv. campestris.
复制标题
XC_0531 编码 c 型细胞色素生物合成蛋白，是野油菜黄单胞菌致病机制所必需的。
DOI：
10.1186/s12866-017-1056-9
复制
发表时间：
2017-06-27
期刊：
影响因子：
4.2
通讯作者：
Lu G
中科院分区：
文献类型：
--
作者：
Chen L;Wang M;Huang L;Zhang Z;Liu F;Lu G
背景：植物病原性野油菜黄单胞菌（Xanthomonas Campestris pv.campestris）是一种革兰氏阴性细菌，是十字花科作物黑腐病的致病菌。许多革兰氏阴性细菌拥有称为 Dsb 的蛋白质家族，它们参与某些周质蛋白质中二硫键的形成。在我们对植物毒力的初步筛选中，我们鉴定了基因 XC_0531，该基因注释了野油菜黄单胞菌 pv 的基因 dsbD。 Campestris (Xcc) 与对宿主植物的毒力有关。结果：在这里，我们发现 XC_0531 编码 DsbD 样蛋白。它的缺失对 DTT 和铜敏感，周质中游离硫醇的积累减少。它的缺失还影响血红素合成、Soret 带的位置和峰 c550 的产生。这表明 XC_0531 与 c 型细胞色素生物发生有关。 XC_0531突变降低了不同碳源（如半乳糖、木糖、麦芽糖、蔗糖和葡萄糖）的利用，减少了胞外多糖（EPS）的产生，降低了胞外酶（蛋白酶、纤维素和淀粉酶）的活性，减慢了Xcc的生长速度，减弱了对植物的毒力。这些结果表明，XC_0531突变引起的这些表型可能是由于呼吸链中c型细胞色素的生物合成和二硫键的形成缺陷所致。我们的工作证实了XC_0531的功能，为科学家们研究细胞色素c生物发生的分子机制、Xcc的发病机制、EPS商业价值的开发以及保护植物免受黑腐病的影响提供理论依据。结论：我们证实了XC_0531基因的功能，该基因编码与c型细胞色素生物发生相关的DsbD样蛋白。该基因通过影响细胞色素c的功能以及可能影响II型分泌系统(T2SS)中蛋白质的二硫键修饰而与植物的毒力相关。
Background:The phytopathogenic Xanthomonas campestris pv.campestris is a gram-negative bacterium and the causal agent of black-rot disease of cruciferous crops. Many gram-negative bacteria possess a family of proteins, called Dsbs, which are involved in disulfide bond formation in certain periplasmic proteins. In our preliminary screening of the virulence to the plants we identified that gene XC_0531 which annotated gene dsbD of Xanthomonas campestris pv. campestris (Xcc) is related to the virulence to the host plants.Results:Here, we found XC_0531 encoded a DsbD like protein. Its deletion is sensitive to DTT and copper, decreased accumulation of free thiols in periplasm. Its deletion also affected heme synthesis, position of Soret band and the production of peak c550. This suggests that XC_0531 is related to c-type cytochromes biogenesis. XC_0531 mutation decreased the utilization of different carbon sources (such as galactose, xylose, maltose, saccharose and glucose), reduced extracellular polysaccharide (EPS) production, decreased extracellular enzyme activities (protease, cellulose and amylase), slowed down growth rate of Xcc and weakened virulence to the plants. These results suggest that these phenotypes caused by XC_0531 mutation is possibly due to deficient biosynthesis of c-type cytochromes in respiration chain and the formation of disulfide bonds. Our work confirmed the function of XC_0531 and provide theory basis for scientists working on molecular mechanisms of cytochrome c biogenesis, pathogenesis of Xcc, development of EPS commercial values and protecting plant from black rot.Conclusion:We confirmed the function of gene XC_0531, which encodes a DsbD like protein, a protein correlated with c-type cytochrome biogenesis. This gene is related to the virulence to plants by affecting funtion of cytochromes c and probably disulfide bonds modification of proteins in type II secretion system (T2SS).