Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: Report of two cases
Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: Report of two cases
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DOI：
10.1023/a:1022548612107
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发表时间：
1998-05-01
影响因子：
3.1
通讯作者：
Veiga, A
中科院分区：
文献类型：
--
作者：
Boada, M;Carrera, M;Veiga, A
目的：台氏酸钻透透明膜进行胚胎活检是植入前遗传学诊断中应用最广泛的方法。相反，我们建议使用一个1.48 μ m的二极管非接触式激光，这是更快，更简单，safer.Methods：激光束被切向引导到胚胎的透明细胞。根据透明膜测量，需要2 - 4次8-22毫秒的连续注射来钻取2例患者（血友病携带者）活检的13个胚胎的透明膜。确定了1例单次妊娠（33.3%着床率）。对不能移植的胚胎进行共培养，发现男性胚胎的囊胚率为66.7%（4/6），异常胚胎的囊胚率为25%（1/4）。
Purpose: The use of Tyrode's acid to drill the zona pellucida for embryo biopsy is the most widely used methodology in preimplantation genetic diagnosis. Instead of this, we propose the use of a 1.48-mu m diode noncontact laser, which is quicker, simpler, and safer.Methods: The laser beam was tangentially guided to the zona pellucida of the embryo. Depending on zona pellucida measurement, two to four consecutive shots of 8-22 msec were necessary to drill the zona pellucida of the 13 embryos biopsied for two patients (hemophilia carriers).Results: Female embryos were replaced into the uterus of the patients (1.5 embryos/replacement). One single pregnancy was established (33.3% implantation rate). Coculture of untransferable embryos showed a blastocyst rate of 66.7% (4/6) for male embryos and 25% (1/4) for abnormal ones.Conclusions: These results demonstrate the safety and usefulness of laser methodology in preimplantation genetic diagnosis.