Plasma‐induced inactivation of Staphylococcus aureus biofilms: The role of atomic oxygen and comparison with disinfectants and antibiotics
Plasma‐induced inactivation of Staphylococcus aureus biofilms: The role of atomic oxygen and comparison with disinfectants and antibiotics
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DOI：
10.1002/ppap.202200147
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发表时间：
2022-10
影响因子：
3.5
通讯作者：
S. Nandula;V. S. Kondeti;Chi Q. Phan;Jianan Wang;M. Penningroth;J. Granick;P. Bruggeman;R. Hunter
中科院分区：
文献类型：
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作者：
S. Nandula;V. S. Kondeti;Chi Q. Phan;Jianan Wang;M. Penningroth;J. Granick;P. Bruggeman;R. Hunter
微生物生物膜因其对抗菌剂的顽固性而备受关注。冷大气等离子体(CAP)是一种很有前途的生物膜修复策略，因为它们产生了活性氧和氮物种(RON)，但CAP-生物膜相互作用的机制尚不清楚。我们评估了处理方式对生物膜灭活的影响，并表明CAP对金黄色葡萄球菌生物膜的杀灭取决于处理条件，包括溶液化学。在干燥处理中，由于等离子体产生的氧通量，生物膜被局部烧蚀。对于盐水淹没的生物膜，虽然我们证明了在较大的处理体积中，在高浓度下会产生ClO−，但在较低的ClO−浓度下，CAP的失活涉及其他反应途径。最后，我们证明了CAP相对于传统抗菌药的有效性，强调了它作为生物被膜治疗方法的前景。
Microbial biofilms are of critical concern because of their recalcitrance to antimicrobials. Cold atmospheric plasmas (CAP) represent a promising biofilm remediation strategy as they generate reactive oxygen and nitrogen species (RONS), but mechanisms underpinning CAP‐biofilm interactions remain unknown. We assess the impact of treatment modality on biofilm inactivation and show that CAP killing ofStaphylococcus aureusbiofilms is dependent on treatment conditions, including solution chemistry. In dry treatments, biofilms are locally ablated due to plasma‐produced O flux. For saline‐submerged biofilms, while we show that ClO−is generated at high concentrations in larger treatment volumes, CAP inactivation at low ClO−concentrations implicates other reaction pathways. Finally, we demonstrate CAP efficacy over conventional antimicrobials, underscoring its promise as a biofilm treatment approach.