In vivo mouse fluorescence imaging for folate-targeted delivery and release kinetics.
In vivo mouse fluorescence imaging for folate-targeted delivery and release kinetics.
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DOI：
10.1364/boe.5.002662
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发表时间：
2014-08-01
影响因子：
3.4
通讯作者：
Low PS
中科院分区：
文献类型：
--
作者：
Tsai EH;Bentz BZ;Chelvam V;Gaind V;Webb KJ;Low PS
许多癌细胞过度表达叶酸受体，这为叶酸靶向荧光成像和靶向抗癌药物的开发提供了机会。我们提出了一种光学成像模式，允许监测和评价药物的输送和释放，通过二硫键还原肿瘤内的第一次在体内。合成了一种近红外叶酸靶向荧光团对，并用于对从活小鼠中的KB细胞生长的异种移植肿瘤进行成像。体内结果与以前的体外研究一致，证实了我们的方法作为药物开发中临床前研究的有效工具的有效性和可行性。
Many cancer cells over-express folate receptors, and this provides an opportunity for both folate-targeted fluorescence imaging and the development of targeted anti-cancer drugs. We present an optical imaging modality that allows for the monitoring and evaluation of drug delivery and release through disulfide bond reduction inside a tumor in vivo for the first time. A near-infrared folate-targeting fluorophore pair was synthesized and used to image a xenograft tumor grown from KB cells in a live mouse. The in vivo results are shown to be in agreement with previous in vitro studies, confirming the validity and feasibility of our method as an effective tool for preclinical studies in drug development.