Gadoxetate disodium-induced tachypnoea and the effect of dilution method: a proof-of-concept study in mice
Gadoxetate disodium-induced tachypnoea and the effect of dilution method: a proof-of-concept study in mice
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DOI：
10.1007/s00330-017-5037-z
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发表时间：
2018-02-01
影响因子：
5.9
通讯作者：
Kiryu, Shigeru
中科院分区：
文献类型：
--
作者：
Akai, Hiroyuki;Yasaka, Koichiro;Kiryu, Shigeru
为了在使用小鼠的实验研究中直接研究钆塞酸二钠的快速呼吸作用，在全身麻醉下确认稳定呼吸状态后，按以下顺序向8只小鼠注射所有试验药物：磷酸盐缓冲盐水（A，对照组）、1.25 mmol/kg钆特醇（B）或钆喷酸葡胺（C）或0.31 mmol/kg钆塞酸二钠（D，E）。实验者并没有对这些物质设盲。A-D组的注射剂量固定为100 μ L，E组为50 μ L。我们持续监测并记录呼吸频率（RR）、外周血氧饱和度（SpO（2））和心率。采用线性混合法比较0 ~ 30 s的时间序列变化，D组和E组RR增加最大（分别为20.6和20.3次/min），与A组（3.36次/min，均P < 0.001）相比显著更大。D组和E组RR变化无差异。B和C组的RR变化较小（分别为0.72和12.4次呼吸/min），与A组无统计学差异。仅C组出现明显心动过缓（P < 0.001）。所有组的SpO（2）均保持恒定，无论采用何种稀释方法，甘氨酯二钠均引起快速呼吸急促，而SpO（2）和心率无显著变化。
To directly investigate the rapid respiratory effect of gadoxetate disodium in an experimental study using mice.After confirming the steady respiratory state under general anaesthesia, eight mice were injected with all test agents in the following order: phosphate-buffered saline (A, control group), 1.25 mmol/kg of gadoteridol (B) or gadopentetate dimeglumine (C), or 0.31 mmol/kg of gadoxetate disodium (D, E). The experimenter was not blinded to the agents. The injection dose was fixed as 100 mu L for Groups A-D and 50 mu L for Group E. We continuously monitored and recorded respiratory rate (RR), peripheral oxygen saturation (SpO(2)), and heart rate. The time-series changes from 0 to 30 s were compared by the linear mixed methodGroups D and E showed the largest RR increase (20.6 and 20.3 breaths/min, respectively) and were significantly larger compared to Group A (3.36 breaths/min, both P < 0.001). RR change of Groups D and E did not differ. RR change of Groups B and C was smaller (0.72 and 12.4 breaths/min, respectively) and did not differ statistically with Group A. Significant bradycardia was observed only in Group C (P < 0.001). SpO(2) was constant in all groups.Gadoxetate disodium causes a rapid tachypnoea without significant change of SpO(2) and heart rate regardless of the dilution method.