Prevotella shahii sp nov and Prevotella salivae sp nov., isolated from the human oral cavity
Prevotella shahii sp nov and Prevotella salivae sp nov., isolated from the human oral cavity
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DOI：
10.1099/ijs.0.02876-0
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发表时间：
2004-05-01
影响因子：
2.8
通讯作者：
Benno, Y
中科院分区：
文献类型：
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作者：
Sakamoto, M;Suzuki, M;Benno, Y
对分离自人口腔的EHS11(T)和EPSA11(T)两株菌株进行表型和生化特征、细胞脂肪酸谱和基于16S rRNA基因序列分析的系统发育定位鉴定。16S rRNA基因序列分析表明，每个分离株均属于普雷沃菌属的一个新种。菌株EHS11(T)与洛氏普雷沃特菌（相似度约95%）有亲缘关系，而菌株EPSA11(T)与氏普雷沃特菌（相似度约94%）有亲缘关系。两株菌株均为专性厌氧，无色素，无孢子形成，无运动，革兰氏阴性棒。菌株EPSA11(T)的细胞脂肪酸组成与P. oris JCM 8540(T)非常相似。另一方面，菌株EHS11(T)的细胞脂肪酸组成与其他普氏菌有显著差异。菌株EHS11(T)的主要脂肪酸为C-18:1 ω - 9c、C-16:0和C-16:0 3-OH，而除P. loescheii JCM 8530(T)外，其他普雷沃氏菌的主要脂肪酸为前C-15:0、iso-C-17:0 3-OH和C(18:1) ω - 9c。P. loescheii JCM 8530(T)的主要脂肪酸为前C-15:0、C-16:0和C(18:1) ω - 9c。DNA-DNA杂交实验结果显示，EHS11(T)和EPSA11(T)菌株与黄颡鱼JCM 8530(T)和牛颡鱼JCM 8540(T)存在基因组差异。在此基础上，提出了两个新的普氏菌种：沙氏普氏菌和唾液普氏普氏菌。沙氏普氏菌和唾液普氏普氏菌的类型菌株分别为EHS11(T) (= JCM 12083（T) = DSM 15611(T)）和EPSA11(T) (= JCM 12084（T) = DSM 15606(T)）。
Two bacterial strains, EHS11(T) and EPSA11(T), which were isolated from the human oral cavity, were characterized in terms of phenotypic and biochemical characteristics, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that each of the isolates belonged to a novel species of the genus Prevotella. Strain EHS11(T) was related to Prevotella loescheii (about 95% similarity), whereas strain EPSA11(T) was related to Prevotella oris (about 94% similarity). Both strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid composition of strain EPSA11(T) was very similar to that of P. oris JCM 8540(T). On the other hand, the cellular fatty acid composition of strain EHS11(T) was significantly different from those of other Prevotella species. The predominant fatty acids in strain EHS11(T) are C-18:1 omega9c, C-16:0 and C-16:0 3-OH, whereas other Prevotella species, except for P. loescheii JCM 8530(T), possess anteiso-C-15:0, iso-C-17:0 3-OH and C(18:1)omega9c. The predominant fatty acids in P. loescheii JCM 8530(T) are anteiso-C-15:0, C-16:0 and C(18:1)omega9c. DNA-DNA hybridization experiments revealed a genomic distinction of strains EHS11(T) and EPSA11(T) from P. loescheii JCM 8530(T) and P. oris JCM 8540(T). On the basis of these data, two novel Prevotella species are proposed: Prevotella shahii sp. nov. and Prevotella salivae sp. nov. The type strains of P. shahii and P. salivae are EHS11(T) (= JCM 12083(T) = DSM 15611(T)) and EPSA11(T) (= JCM 12084(T) = DSM 15606(T)), respectively.