Long noncoding RNA PCAT-14 induces proliferation and invasion by hepatocellular carcinoma cells by inducing methylation of miR-372.
Long noncoding RNA PCAT-14 induces proliferation and invasion by hepatocellular carcinoma cells by inducing methylation of miR-372.
复制标题
DOI：
10.18632/oncotarget.16260
复制
发表时间：
2017-05-23
期刊：
影响因子：
--
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Wang Y;Hu Y;Wu G;Yang Y;Tang Y;Zhang W;Wang K;Liu Y;Wang X;Li T
长链非编码RNA（lncRNA）通过诱导CpG岛甲基化以沉默靶基因来调节肿瘤发生。在这里，我们发现lncRNA PCAT-14在肝细胞癌（HCC）患者中过表达，并且与手术后的不良预后相关。我们的研究结果表明，PCAT-14促进肝癌细胞的增殖，侵袭和细胞周期阻滞。此外，PCAT-14通过诱导miR-372启动子的甲基化来抑制miR-372表达。同时，miR-372消除了PCAT-14对HCC细胞增殖、侵袭和细胞周期的影响。此外，PCAT-14通过miR-372调节ATAD 2的表达和Hedgehog通路的激活。这些结果表明PCAT-14在HCC中起重要作用，并可能作为一个新的预后因子和治疗靶点。
Long non-coding RNAs (lncRNAs) regulate oncogenesis by inducing methylation of CpG islands to silence target genes. Here we show that the lncRNA PCAT-14 is overexpressed in patients with hepatocellular carcinoma (HCC), and is associated with a poor prognosis after surgery. Our results demonstrate that PCAT-14 promotes proliferation, invasion, and cell cycle arrest in HCC cells. In addition, PCAT-14 inhibits miR-372 expression by inducing methylation of the miR-372 promoter. Simultaneously, miR-372 eliminates the effects of PCAT-14 on proliferation, invasion, and cell cycle in HCC cells. Moreover, PCAT-14 regulates expression of ATAD2 and activation of the Hedgehog pathway via miR-372. These findings indicate that PCAT-14 plays an important role in HCC, and may serve as a novel prognostic factor and therapeutic target.