Aberrant Expression of USP22 is Associated With Liver Metastasis and Poor Prognosis of Colorectal Cancer
Aberrant Expression of USP22 is Associated With Liver Metastasis and Poor Prognosis of Colorectal Cancer
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DOI：
10.1002/jso.21802
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发表时间：
2011-03-01
影响因子：
2.5
通讯作者：
Dong, Xin-Shu
中科院分区：
文献类型：
--
作者：
Liu, Yan-Long;Yang, Yan-Mei;Dong, Xin-Shu
背景资料：本研究旨在探讨泛素羧基末端水解酶22（ubiquitin carboxyl-terminal hydrolase 22，USP 22）在大肠癌中的表达及其临床意义。Western blot和免疫组化（IHC）在43个CRC和非癌匹配组织中。结果：在43例结直肠癌组织中，USP 22蛋白在mRNA和蛋白水平的表达均显著高于癌旁组织（P < 0.0001）;从正常粘膜到腺瘤到原发癌（P < 0.0001）和从原发癌到肝转移（P = 0.021），核USP 22表达显著增加。在192例常规大肠癌组织中，USP 22阳性表达率为54.16%。值得注意的是，高USP 22表达与较短的疾病特异性生存期（P < 0.0001）和较短的无病生存期（P < 0.0001）显著相关。结论：USP 22可能是结直肠癌患者预后的独立预测因子，USP 22的异常表达可能在结直肠癌的发生发展及肝转移中起重要作用。外科肿瘤学杂志2011：103：283-289. (C)2010 Wiley-Liss，Inc.
Background: The present study was aimed at clarifying the expression of ubiquitin carboxyl-terminal hydrolase 22 (USP22), a novel deubiquitinating enzyme gene, in colorectal cancer (CRC) and its clinical significance.Methods: USP22 expression was detected with quantitative RT-PCR. Western blot, and immunohistochemistry (IHC) in 43 CRCs and non-cancerous matched tissues. Furthermore, USP22 protein expression was analyzed in 192 CRC tumors by IHC to evaluate the association with survival.Results: In 43 paired fresh tissues, the expression level of USP22 was significantly higher in primary CRCs than that in the paired non-cancerous tissues at both mRNA and protein levels (P < 0.0001). Nuclear USP22 expression significantly increased from normal mucosa through adenoma to primary carcinoma (P < 0.0001) and from primary carcinoma to liver metastasis (P = 0.021). The incidence of positive USP22 expression was 54.16% in 192 conventional CRC tissues. Notably, high USP22 expression was significantly associated with shorter disease-specific survival (P < 0.0001) and shorter disease-free survival (P < 0.0001). Cox regression analysis showed USP22 was an independent prognostic parameter for CRC patients.Conclusion: USP22 might be an independent predictive factor for CRC prognosis and aberrant expression of USP22 may play an essential role in colorectal carcinogenesis and liver metastasis. J. Surg. Oncol. 2011:103:283-289. (C) 2010 Wiley-Liss, Inc.