SELECTIVE ACTIVATION OF EXTRATHYMIC T-CELLS IN THE LIVER BY GLYCYRRHIZIN
SELECTIVE ACTIVATION OF EXTRATHYMIC T-CELLS IN THE LIVER BY GLYCYRRHIZIN
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DOI：
10.1007/bf02171049
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发表时间：
1992-10-01
期刊：
影响因子：
--
通讯作者：
ABO, T
中科院分区：
文献类型：
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作者：
KIMURA, M;WATANABE, H;ABO, T
T细胞分化的胸腺外途径最近在肝、肠和大网膜被证明。本文研究了植物提取物甘草酸(GL)对小鼠肝脏胸腺外T细胞的影响。采用抗LFA-1单抗与抗TCR或-CD3单抗联合检测胸腺外T细胞的方法。单次注射和多次注射GL不仅增加了肝脏MNC的总数，而且增加了中间TCR细胞的比例，TCR细胞是肝脏中唯一的胸腺外T细胞。与其他受试试剂(如淋巴毒素和雌激素)不同，GL激活胸腺外T细胞并同时导致严重的胸腺萎缩，GL不影响胸腺中的正常T细胞。提示肝组织胸腺外T细胞的选择性激活可能与GL的临床作用密切相关。
Extrathymic pathways for T cell differentiation were recently demonstrated in the liver, intestine and omentum. In this study, glycyrrhizin (GL), a plant extract was investigated as to its effect on extrathymic T cells in the liver of mice. A new method using anti-LFA-1 mAb in conjunction with anti-TCR or -CD3 mAbs to sensitively identify such extrathymic T cells is included. Single injection and repeated injections of GL increased not only the number of total hepatic MNC but also the proportion of intermediate TCR cells, which are extrathymic T cells uniquely seen in the liver. In contrast to other tested reagents (e.g., lymphotoxin and estrogen) that activated the extrathymic T cells and simultaneously induced profound thymic atrophy, GL did not affect regular T cells in the thymus. The present results suggest that the selective activation of extrathymic T cells in the liver might be intimately related to the clinical effects of GL.