Primary avian tendon cells in culture. An improved system for understanding malignant transformation
Primary avian tendon cells in culture. An improved system for understanding malignant transformation
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培养中的原代禽肌腱细胞。
DOI：
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发表时间：
1978
影响因子：
7.8
通讯作者：
M. J. Bissell
中科院分区：
文献类型：
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作者：
Richard I Schwarz;Deborah A Farson;Whai;M. J. Bissell
原代禽腱(PAT)细胞在培养过程中保持分化状态，可被Rous肉瘤病毒快速转化。根据形态、2-脱氧葡萄糖摄取率增加和失去密度依赖性生长控制的标准，PAT细胞和它们分化较低的对应物-鸡胚成纤维细胞一样转化。此外，PAT细胞在转化过程中产生的胶原蛋白的百分比下降了一个数量级，从23%下降到2.5%，但不受转化缺陷突变体的病毒复制的影响。正常和转化的PAT细胞对各种环境因素的反应性在转化过程中发生了巨大的变化。正常的PAT细胞对抗坏血酸和高细胞密度的反应是通过将胶原合成水平从5%提高到23%来实现的。转化后的PAT细胞完全没有反应。这些和以前报道的结果使我们假设，细胞用来维持分化状态的正常调控机制的崩溃与恶性转化的开始有关或负责。
Primary avian tendon (PAT) cells which maintain their differentiated state in culture are rapidly transformed by Rous sarcoma virus. By criteria of morphology, increased rate of 2-deoxyglucose uptake, and loss of density dependent growth control, PAT cells transform as well as their less differentiated counterpart, chick embryo fibroblasts. In addition, the percentage of collagen produced by PAT cells drops on transformation by an order of magnitude, from 23 to 2.5%, but is unaffected by viral replication of a transformation-defective mutant. The responsiveness of normal and transformed PAT cells to various environmental factors changes dramatically upon transformation. Normal PAT cells respond to the presence of ascorbate and high cell density by raising the level of collagen synthesis from 5 to 23%. Transformed PAT cells are totally unresponsive. These and previously reported results lead us to postulate that the break-down in the normal regulatory mechanisms used by the cell to maintain the differentiated state is related to or is responsible for the onset of malignant transformation.