High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats
High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats
复制标题
高谷物喂养改变山羊盲肠细菌微生物群组成和发酵，导致盲肠粘膜损伤
DOI：
10.1017/s0007114514000993
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发表时间：
2014-08-14
影响因子：
3.6
通讯作者：
Mao, Shengyong
中科院分区：
文献类型：
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作者：
Liu, Junhua;Xu, Tingting;Mao, Shengyong
高谷物（HG）喂养对盲肠细菌微生物群组成和发酵以及粘膜健康的影响在很大程度上是未知的。在本研究中，10只公山羊被随机分配到饲喂干草饮食（0%谷物; n 5）或饲喂HG饮食（65%谷物; n 5）的组中，以观察盲肠中细菌群落组成和粘膜形态的变化。饲喂7周后，HG组盲肠pH值显著降低（P<0.001），盲肠粘膜总挥发性脂肪酸和脂多糖（LPS）浓度显著升高（P<0.001 ~ P<0.004）。16 S核糖体RNA基因的焦磷酸测序显示，与干草饮食相比，HG喂养增加了（P= 0·001至P= 0·009）盲肠腔和盲肠粘膜中优势属Turicibacter和Clostridium的丰度，并降低了（P< 0·001至P< 0·009）腔中拟杆菌和粘膜中粘菌的比例。此外，饲喂高糖日粮的山羊盲肠粘膜上皮细胞损伤明显，IL-1β、IL-6、IL-12和IFN-γ的相对mRNA表达量显著上调（P<0.001 ~ P<0.025）。相关性分析表明，盲肠pH值，LPS浓度和粘膜相关的微生物群丰度在HG喂养期间的改变可能部分有助于局部炎症。总的来说，这些结果提供了深入了解盲肠细菌种群的适应性反应，HG喂养山羊，并揭示流入盲肠的发酵底物可能会导致微生物组成的显着改变，并在盲肠功能障碍中发挥重要作用。
The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown. In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0 % grain; n 5) or a group fed a HG diet (65 % grain; n 5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum. After 7 weeks of feeding, the HG diet decreased the caecal pH (P< 0·001) and increased (P< 0·001 to P< 0·004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS). Pyrosequencing of the 16S ribosomal RNA gene revealed that HG feeding increased (P= 0·001 to P= 0·009) the abundance of predominant genera Turicibacter and Clostridium in the caecal lumen and in the caecal mucosa and decreased (P< 0·001 to P< 0·009) the proportion of Bacteroides in the lumen and Mucispirillum in the mucosa compared with the hay diet. Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (P< 0·001 to P< 0·025) of the relative mRNA expression of IL-1β, IL-6, IL-12 and interferon-γ (IFN-γ) in the caecal mucosa. The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation. Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.