Evolutionary conservation of complexins: from choanoflagellates to mice
Evolutionary conservation of complexins: from choanoflagellates to mice
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DOI：
10.15252/embr.201540305
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发表时间：
2015-10
期刊：
影响因子：
7.7
通讯作者：
Xiaofei Yang;Jimin Pei;Yea Jin Kaeser-Woo;Taulant Bacaj;N. Grishin;T. Südhof
中科院分区：
文献类型：
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作者：
Xiaofei Yang;Jimin Pei;Yea Jin Kaeser-Woo;Taulant Bacaj;N. Grishin;T. Südhof
复合蛋白是突触 SNARE 复合物结合蛋白，与突触结合蛋白配合激活 Ca2+ 刺激的、突触结合蛋白依赖的突触小泡胞吐作用，并钳制自发的、不依赖突触结合蛋白的突触小泡胞吐作用。在这里，我们表明，络合蛋白序列在一些非后生动物单细胞生物和所有后生动物中是保守的，这表明络合蛋白是早于后生动物进化的后生动物的普遍特征。我们发现，来自 Nematostella vectensis 的络合蛋白（一种在后生动物进化中与哺乳动物相距甚远的刺胞动物海葵）在功能上取代了小鼠络合蛋白激活 Ca2+ 触发的胞吐作用，但无法抑制自发的胞吐作用。因此，络合蛋白的激活功能可能在后生动物的进化过程中得到保留。
Complexins are synaptic SNARE complex‐binding proteins that cooperate with synaptotagmins in activating Ca2+‐stimulated, synaptotagmin‐dependent synaptic vesicle exocytosis and in clamping spontaneous, synaptotagmin‐independent synaptic vesicle exocytosis. Here, we show that complexin sequences are conserved in some non‐metazoan unicellular organisms and in all metazoans, suggesting that complexins are a universal feature of metazoans that predate metazoan evolution. We show that complexin from Nematostella vectensis, a cnidarian sea anemone far separated from mammals in metazoan evolution, functionally replaces mouse complexins in activating Ca2+‐triggered exocytosis, but is unable to clamp spontaneous exocytosis. Thus, the activating function of complexins is likely conserved throughout metazoan evolution.