Differentiation of intraspecific groups within anastomosis group 1 of Rhizoctonia solani using ribosomal DNA internal transcribed spacer and isozyme comparisons
Differentiation of intraspecific groups within anastomosis group 1 of Rhizoctonia solani using ribosomal DNA internal transcribed spacer and isozyme comparisons
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使用核糖体 DNA 内转录间隔区和同工酶比较区分立枯丝核菌吻合组 1 内的种内组
DOI：
10.1080/07060669309501923
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发表时间：
1993
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影响因子：
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通讯作者：
J. Sinclair
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作者：
Z. L. Liu;J. Sinclair
采用DNA限制性片段分析和同工酶分析方法，研究了61个水稻立枯丝核菌（Rhizoctoniasolanisomagonaesthesia group 1，AG 1）菌株的遗传多样性。用聚合酶链反应（PCR）扩增每个分离物的编码核核糖体RNA内转录间隔区（ITS）的基因区域，包括5.8 S rDNA。基于分离株的独特DNA限制性内切酶消化模式，将分离株的6个遗传上不同的种内组（ISG）区分为：ISG 1A（10个分离株）、IB（4个分离株）、1C（15个分离株）、ID（5个分离株）、IE（2个分离株）和IF（25个分离株）。PCR扩增的DNA长度ITS-5.8 S的6个ISG的变化，并估计在0.69和0.73之间的酶对（kb）。构建了ITS-5.8SrDNA区域内6个ISG的DNA限制性内切酶图谱。这些ISG共享一些位点，但在几个Haelll、Mbo\和Taq\位点上彼此不同。小的DNA插入或缺失似乎发生在各种ISG之间。这6个群体的线粒体小rDNA均为0.55kb，其中Haell和Hint各1个。18 SrDNA为1.8kb，有6种酶的22个共同限制性位点。许多同工酶等位基因和位点被鉴定为每个ISG的同工酶标记，并为该组所有成员所共有。六个DNA分化的ISG与三个先前描述的AG 1（AG 1-IA，-IB和-IC）的形态学为基础的亚组的关系进行了讨论。利用酶切位点和同工酶标记，对该菌AG 1内的ISG进行了鉴定. solani提供了新的字符，以提高复杂的遗传不同的实体内的识别和推断种内的AG 1菌株之间的关系。
Genetic diversity among 61 isolates of Rhizoctonia solani anastomosis group 1 (AG 1) was studied by DNA restriction fragment and isozyme analyses. Gene regions encoding nuclear ribosomal RNA internal transcribed spacers (ITS), including 5.8 S rDNA, were amplified with polymerase chain reaction (PCR) for each isolate. Six genetically-distinct intraspecific groups (ISGs) of isolates were differentiated based on their unique DNA restriction digest patterns by 1 I endonucleases: ISG 1A (10 isolates), IB (four isolates), 1C (15 isolates), ID (five isolates), IE (two isolates), and IF (25 isolates). PCR-amplified DNA lengths of ITS-5.8 S for the six ISGs varied and were estimated between 0.69 and 0.73 kilobase pairs (kb). DNA restriction maps for the six ISGs in the ITS-5.8 S rDNA region were constructed. These ISGs shared some sites but differed from one another for several Haelll, Mbo\and Taq\sites. Small DNA insertions or deletions appeared to occur among the various ISGs. The six groups had the same mitochondrial small rDNA of 0.55 kb with one Haelll and one Hint! site, and the same nuclear 18 S rDNA of 1.8 kb with 22 restriction sites of six enzymes in common. Many isozyme alleles and loci were identified as isozyme markers for each ISG and were shared by all members in the group. The relationships of the six DNA-differentiated ISGs with the three previously described morphology-based subgroups of AG 1 (AG 1-IA,-IB, and-IC) are discussed. The DNA restriction sites and isozyme markers identified for the ISGs within AG 1 of R. solani provide new characters for improving the identification of genetically distinct entities within the complex and for inferring intraspecific relationships among isolates in AG 1.
Minoru Ide、Koji Makino、Akihiko Uchibashira、Mitsuyasu Mori：EAR 研究日本 18。（1987）
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