Photochemical tissue bonding: A promising technique for peripheral nerve repair
Photochemical tissue bonding: A promising technique for peripheral nerve repair
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DOI：
10.1016/j.jss.2007.01.028
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Redmond, Robert W.
中科院分区：
文献类型：
--
作者：
Johnson, T. Shane;O'Neill, Anne C.;Redmond, Robert W.
背景光化学组织粘合（PTB）是一种新型的组织修复技术，它利用可见光和光敏染料交联组织表面的蛋白质。该技术已在许多组织修复模型中得到成功证明。理想的神经修复技术应是无创的，并避免在修复部位放置异物。神经外膜适合于光化学修复，因为它薄、半透明并且具有相对高的胶原蛋白含量。本研究旨在确定PTB是否可以成功应用于周围神经修复模型。材料和方法。对40只Sprague道利大鼠进行坐骨神经横断。然后将动物随机分配至4个治疗组：神经外膜缝合修复、神经外膜套囊联合PTB、单独神经外膜套囊和无修复。每隔10天使用步行轨迹分析和坐骨神经功能指数计算评估功能恢复。术后90天处死动物，取坐骨神经进行组织学和组织形态计量学检查。修复后90天时，缝合修复组和PTB神经外膜袖套组的功能恢复无显著差异（-70.6 \ 17.8 vs-76.9 \ 10.3，P = 0.64）。组织学显示所有修复技术均具有良好的轴突再生。组织形态学分析发现修复组之间无显著差异。这项研究表明，周围神经可以成功地修复使用光化学组织粘合技术与目前的金标准所取得的结果相似。随着进一步的发展和完善PTB可能被证明是一个有用的工具，在周围神经修复。(c)2007年爱思唯尔公司All rights reserved.
Background. Photochemical tissue bonding (PTB) is a novel tissue repair technique that uses visible light and a photosensitizing dye to crosslink proteins on tissue surfaces. This technique has been successfully demonstrated in a number of tissue repair models. An ideal nerve repair technique would be atraumatic and avoid placement of foreign bodies at the repair site. The epineurium is suited to photochemical repair as it is thin, translucent and has a relatively high collagen content. This study was designed to determine if PTB could be successfully applied in a peripheral nerve repair model.Material and methods. Forty Sprague Dawley rats underwent transection of the sciatic nerve. Animals were then randomized to four treatment groups; epineurial suture repair, epineurial cuff with PTB, epineurial cuff alone, and no repair. Functional recovery was assessed at 10 day intervals using walking track analysis and sciatic function index calculations. At 90 days postoperatively animals were sacrificed and sciatic nerves harvested for histology and histomorphometry.Results. Functional recovery in the suture repair and epineural cuff with PTB groups were not significantly different (-70.6 \ 17.8 versus -76.9 \ 10.3, P = 0.64) at 90 days postrepair. Histology showed good axonal regeneration with all repair techniques. Histomorphometric analysis found no significant difference between the repair groups.Conclusions. This study illustrates that peripheral nerves can be successfully repaired using a photochemical tissue bonding technique with results similar to those achieved with the current gold standard. With further development and refinement PTB may prove a useful tool in peripheral nerve repair. (c) 2007 Elsevier Inc. All rights reserved.