Optimization of the extraction of paclitaxel from Taxus baccata L. by the use of microwave energy.
Optimization of the extraction of paclitaxel from Taxus baccata L. by the use of microwave energy.
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利用微波能量从红豆杉中提取紫杉醇的优化。
DOI：
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发表时间：
2004
影响因子：
3.1
通讯作者：
L. Dolatyari
中科院分区：
文献类型：
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作者：
M. Talebi;A. Ghassempour;Z. Talebpour;A. Rassouli;L. Dolatyari
采用微波辅助萃取法从红豆杉针叶中提取紫杉醇。生长在伊朗的栖息地。将样品浸入甲醇-水混合物中，在密闭容器系统中用微波照射。根据浸提时间、温度、水中甲醇浓度（v/v）和样品克数与10 mL溶剂的比例等参数，采用析因设计方法对该方法进行评价。统计学处理结果表明，所选参数除浸提时间外均具有显著性。最佳条件将是1.5克样品在10毫升溶剂（90%甲醇），95摄氏度的提取温度，和提取时间为7分钟。提取物已被分析，通过反相高效液相色谱法与紫外检测器（LC/UV）在227 nm处进行定量。采用液相色谱-串联质谱法（LC-MS/MS）进行确认。与常规溶剂萃取（CSE）相比，拟定MAE方法的主要优点是时间（7 min vs 16 h）和溶剂消耗量（20 mL vs 150 mL）显著减少。MAE程序产生的浸提液可直接进行分析，无需任何初步净化或溶剂交换步骤。两种提取方法均显示RSD低于10%，并且紫杉醇的回收率相当（87 - 92%）。
A simple and rapid microwave-assisted extraction (MAE) procedure was developed and optimized for the extraction of paclitaxel (Taxol) from the needles of yew trees Taxus baccata L. grown in Iranian habitats. The samples, immersed in a methanol-water mixture, were irradiated with microwaves in a closed-vessel system. The method was evaluated using a factorial design approach based on parameters such as extraction time, temperature, methanol concentration in water (v/v), and the ratio of grams of sample to 10 mL of solvent. Statistical treatment of the results revealed that the selected parameters were all significant except the extraction time. Optimum conditions would be 1.5 g samples in 10 mL solvent (90% methanol), an extraction temperature of 95 degrees C, and an extraction time of 7 min. The extracts has been analyzed by reverse-phase high-performance liquid chromatography with UV detection (LC/UV) at 227 nm for quantification. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for confirmation. The main advantage of the proposed MAE method versus conventional solvent extraction (CSE) are the considerable reductions in time (7 min versus 16 h) and in solvent consumption (20 mL versus 150 mL). The MAE procedure yielded extracts that could be analyzed directly without any preliminary clean-up or solvent exchange steps. Both extraction methods show RSDs lower than 10% and lead to comparable recoveries of paclitaxel (87-92%).
影响因子：
5.1
作者：
Baloglu, E;Kingston, DGI
通讯作者：
Kingston, DGI