Biocompatibility index of antiseptic agents by parallel assessment of antimicrobial activity and cellular cytotoxicity
Biocompatibility index of antiseptic agents by parallel assessment of antimicrobial activity and cellular cytotoxicity
复制标题
DOI：
10.1093/jac/dkn125
复制
发表时间：
2008-06-01
影响因子：
5.2
通讯作者：
Kramer, Axel
中科院分区：
文献类型：
--
作者：
Mueller, Gerald;Kramer, Axel
目的：为了评估抗菌剂的适用性，必须考虑到杀菌活性和细胞毒性效应，以获得生物相容性antibiotics agents.Methods：我们定义的生物相容性指数（BI）通过测量对测试生物大肠杆菌和金黄色葡萄球菌的抗菌活性，并在平行，对培养的小鼠成纤维细胞的细胞毒性。测试的防腐剂为苯扎氯铵（BAC）、氯化十六烷基吡啶（CPC）、二葡萄糖酸氯己定（CHX）、温和银蛋白（MSP）、二盐酸奥替尼啶（OCT）、聚双胍（PHMB）、聚维酮碘溶液[PVP-I（s）]、聚维酮碘软膏[PVP-I（o）]、硝酸银（AgNO 3）、银（I）、磺胺嘧啶（SSD）和三氯生（TRI）。在含有10%胎牛血清的细胞培养基存在下，在37 ℃下暴露30分钟进行测定。所得无量纲BI定义为50%鼠成纤维细胞被损伤的浓度与产生至少3log（10）（99.9%）减少的杀微生物效果的比率。对大肠杆菌的毒力为OCT > PHMB > CHX > PVP- I（o）> PVP-I（s）> BAC > CPC > TRI > MSP;金黄色葡萄球菌的抗肿瘤活性顺序为OCT > PHMB > CHX > CPC > PVP- I（o）> BAC > PVP（s）> TRI > MSP。OCT和PHMB的BI值大于1，是最合适的抗菌剂。结论：本研究提出的BI值可作为抗菌剂临床应用评价的一个有用工具。
Objectives: To assess the suitability of an antiseptic agent, both the microbicidal activity and the cytotoxic effect must be taken into consideration to derive biocompatible antibacterial agents.Methods: We defined the biocompatibility index (BI) by measuring the antibacterial activity against the test organisms Escherichia coli and Staphylococcus aureus and, in parallel, the cytotoxicity on cultured murine fibroblasts. The antiseptic agents tested were benzalkonium chloride (BAC), cetylpyridinium chloride (CPC), chlorhexidine digluconate (CHX), mild silver protein (MSP), octenidine dihydrochloride (OCT), polyhexamethylene biguanide (PHMB), povidone iodine in solution [PVP-I(s)], povidone iodine in ointment [PVP-I(o)], silver nitrate (AgNO3), silver (I) sulfadiazine (SSD) and triclosan (TRI). Assays were carried out for 30 min of exposure at 37 degrees C in the presence of cell culture medium containing 10% fetal bovine serum. The resulting dimensionless BI was defined as the ratio of the concentration at which 50% of the murine fibroblasts are damaged and the microbicidal effect producing at least 3 log(10) (99.9%) reduction.Results: The resulting rank ordering of BI for the ratio of fibroblast cytotoxicity to E. coli toxicity was OCT > PHMB > CHX > PVP- I(o) > PVP-I(s) > BAC > CPC > TRI > MSP and that to S. aureus was OCT > PHMB > CHX > CPC > PVP- I(o) > BAC > PVP(s) > TRI > MSP. OCT and PHMB were the most suitable agents with a BI greater than 1.Conclusions: The BI presented may be a useful tool to evaluate antiseptic agents for use in clinical practice.