Host-microbiota interactions and responses to grass carp reovirus infection in Ctenopharyngodon idellus
Host-microbiota interactions and responses to grass carp reovirus infection in Ctenopharyngodon idellus
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草鱼宿主微生物群相互作用及对草鱼呼肠孤病毒感染的反应
DOI：
10.1111/1462-2920.15330
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发表时间：
2021
影响因子：
5.1
通讯作者：
Yan Qingyun
中科院分区：
文献类型：
--
作者：
Xiao Fanshu;Liao Lanjie;Xu Qiaoqing;He Zhili;Xiao Tiaoyi;Wang Jianjun;Huang Jie;Yu Yuhe;Wu Bo;Yan Qingyun
肠道微生物群可以促进宿主防御疾病，但病毒感染期间鱼类-微生物群的相互作用及其潜在机制知之甚少。研究了草鱼呼肠孤病毒（GCRV）感染对草鱼肠道菌群的影响。我们发现，具有严重出血症状（G7）的GCRV感染组显示出相当不同的肠道微生物群，特别是具有异常高丰度的革兰氏阴性厌氧菌。它还显示出最低（p< 0.05）的α多样性，但具有更高的肠道扩散生态过程（28.8%），证实了病毒感染后肠道微生物群的生态失调。有趣的是，NOD样受体（NLR），Toll样受体（TLR）和脂多糖（LPS）刺激基因的信号通路在G7中显著富集（q值< 0.01），这也与核心肠道微生物属Cetobacterium和Acinetobacter显著相关（p< 0.01）。结果表明，C. GCRV通过LPS相关的NLR和TLR途径，可加重宿主的炎症反应。这项研究推进了我们对鱼类免疫力与受病毒挑战的肠道微生物群之间相互作用的理解;它还为肠道微生物群的帮助下鱼类疾病的生态防御提供了新的见解。
Gut microbiota could facilitate host to defense diseases, but fish–microbiota interactions during viral infection and the underlying mechanism are poorly understood. We examined interactions and responses of gut microbiota to grass carp reovirus (GCRV) infection inCtenopharyngodon idellus, which is the most important aquaculture fish worldwide. We found that GCRV infection group with serious haemorrhagic symptoms (G7s) showed considerably different gut microbiota, especially with an abnormally high abundance of gram‐negative anaerobicCetobacterium somerae. It also showed the lowest (p< 0.05) alpha‐diversity but with much higher ecological process of homogenizing dispersal (28.8%), confirming a dysbiosis of the gut microbiota after viral infection. Interestingly, signaling pathways of NOD‐like receptors (NLRs), toll‐like receptors (TLRs), and lipopolysaccharide (LPS) stimulation genes were significantly (q‐value < 0.01) enriched in G7s, which also significantly (p< 0.01) correlated with the core gut microbial genera ofCetobacteriumandAcinetobacter. The results suggested that an expansion ofC. someraeinitiated by GCRV could aggravate host inflammatory reactions through the LPS‐related NLRs and TLRs pathways. This study advances our understanding of the interplay between fish immunity and gut microbiota challenged by viruses; it also sheds new insights for ecological defense of fish diseases with the help of gut microbiota.