Serum vaspin concentrations in human obesity and type 2 diabetes
Serum vaspin concentrations in human obesity and type 2 diabetes
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DOI：
10.2337/db07-1045
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发表时间：
2008-02-01
期刊：
影响因子：
7.7
通讯作者：
Blueher, Matthias
中科院分区：
文献类型：
--
作者：
You, Byung-Soo;Kloeting, Nora;Blueher, Matthias
APPLICATIVE-Vaspin被鉴定为具有胰岛素增敏作用的脂肪因子，其主要从2型糖尿病大鼠模型的内脏脂肪组织分泌。我们最近发现脂肪组织中vaspin mRNA的表达与肥胖和葡萄糖代谢的参数有关。然而，在人类肥胖和2型糖尿病的vaspin血清浓度的调节是unknown.研究设计和方法-为测量vaspin血清浓度，我们开发了一种酶联免疫吸附试验（ELISA）。使用这种ELISA，我们在一项横断面研究中评估了187名具有广泛肥胖、体脂分布、胰岛素敏感性和葡萄糖耐量的受试者以及60名具有正常葡萄糖耐量（NGT）、葡萄糖耐量受损（IGT）、或2型糖尿病之前和之后4周的体育锻炼计划。结果-女性受试者的Vaspin血清浓度显著高于男性受试者。NGT和2型糖尿病患者之间的循环vaspin没有差异。在正常葡萄糖耐受组中，循环vaspin与BMI和胰岛素敏感性显著相关。此外，体育锻炼4周导致循环vaspin levels.CONCLUSIONS-We发现循环vaspin的性别二型性。血清vaspin浓度升高与肥胖和胰岛素敏感性受损有关，而2型糖尿病似乎消除了循环vaspin增加、体重增加和胰岛素敏感性降低之间的相关性。低循环vaspin与高健身水平相关，而未经训练的个体的体育训练导致vaspin血清浓度增加。
OBJECTIVE-Vaspin was identified as an adipokine with insulin-sensitizing effects, which is predominantly secreted from visceral adipose tissue in a rat model of type 2 diabetes. We have recently shown that vaspin mRNA expression in adipose tissue is related to parameters of obesity and glucose metabolism. However, the regulation of vaspin serum concentrations in human obesity and type 2 diabetes is unknown.RESEARCH DESIGN AND METHODS-For the measurement of vaspin serum concentrations, we developed an enzyme-linked immunosorbent assay (ELISA). Using this ELISA, we assessed circulating vaspin in a cross-sectional study of 187 subjects with a wide range of obesity, body fat distribution, insulin sensitivity, and glucose tolerance and in 60 individuals with normal glucose tolerance (NGT), impaired glucose tolerance (IGT), or type 2 diabetes before and after a 4-week physical training program.RESULTS-Vaspin serum concentrations were significantly higher in female compared with male subjects. There was no difference in circulating vaspin between individuals with NGT and type 2 diabetes. In the normal glucose-tolerant group, circulating vaspin significantly correlated with BMI and insulin sensitivity. Moreover, physical training for 4 weeks resulted in significantly increased circulating vaspin levels.CONCLUSIONS-We found a sexual dimorphism in circulating vaspin. Elevated vaspin serum concentrations are associated with obesity and impaired insulin sensitivity, whereas type 2 diabetes seems to abrogate the correlation between increased circulating vaspin, higher body weight, and decreased insulin sensitivity. Low circulating vaspin correlates with a high fitness level, whereas physical training in untrained individuals causes increased vaspin serum concentrations.