Cloning of the Lactococcus lactis adhE gene, encoding a multifunctional alcohol dehydrogenase, by complementation of a fermentative mutant of Escherichia coli
Cloning of the Lactococcus lactis adhE gene, encoding a multifunctional alcohol dehydrogenase, by complementation of a fermentative mutant of Escherichia coli
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DOI：
10.1128/jb.180.12.3049-3055.1998
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发表时间：
1998-06-01
影响因子：
3.2
通讯作者：
Israelsen, H
中科院分区：
文献类型：
--
作者：
Arnau, J;Jorgensen, F;Israelsen, H
乳酸乳球菌adhE基因，它编码一个多功能的乙醇脱氢酶，已被克隆和表征。通过筛选L.乳酸菌基因组文库中的大肠杆菌的发酵突变体，并选择厌氧生长的能力。对获得的克隆进行进一步分析，可以克隆整个adhE基因序列。adhE在L.乳酸菌在厌氧条件下，特别是在含葡萄糖的培养基中，表现出转录水平的诱导。构建的突变菌株在厌氧条件下产生减少量的乙醇。与L. lactis基因作为探针，在其他工业相关的乳酸菌中发现了adhE同源物。
The Lactococcus lactis adhE gene, which encodes a multifunctional alcohol dehydrogenase, has been cloned and characterized. A DNA fragment encoding the putative alcohol dehydrogenase domain of the AdhE protein was cloned by screening an L. lactis genomic library in a fermentative mutant of Escherichia coli and selecting for the ability to grow anaerobically. Further analysis of the clone obtained allowed the cloning of the entire adhE gene sequence. Analysis of adhE expression in L. lactis during anaerobiosis showed induction at the transcriptional level, especially in medium containing glucose. Constructed mutant strains produced reduced amounts of ethanol under anaerobic conditions. With the L. lactis gene as a probe, adhE homologs were found in other industrially relevant lactic acid bacteria.